Department of Respiratory and Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, P. R. China.
Oncology Department, The Affiliated Hospital of Qingdao University, Qingdao, P. R. China.
Bioengineered. 2022 Mar;13(3):6711-6718. doi: 10.1080/21655979.2022.2025707.
Circular RNA (circRNA) hsa_circ_0077837 inhibits colorectal cancer. Our research studied the participation of hsa_circ_0077837 in non-small cell lung cancer (NSCLC). Hsa_circ_0077837 and phosphatase and tensin homolog (PTEN) expression in cancer and paired non-cancer tissues from a total of 64 NSCLC patients were studied with RT-qPCR. To evaluate the prognostic value of hsa_circ_0077837 for NSCLC, these 64 patients were monitored for 5 years. Expression of PTEN in NSCLC cells with hsa_circ_0077837 overexpression was determined by RT-qPCR and Western blot. The methylation of PTEN gene in cells transfected with hsa_circ_0077837 expression vector was analyzed by methylation specific PCR (MSP). The roles of hsa_circ_0077837 and PTEN in NSCLC cell proliferation were evaluated using cell apoptosis assay. Our data showed that hsa_circ_0077837 was upregulated in NSCLC and predicted poor survival. Besides, hsa_circ_0077837 expression level was higher in 36 advanced cases (stage III and IV) than in 28 early-stage cases (stage I and II). Hsa_circ_0077837 was inversely correlated with PTEN across cancer tissues. In NSCLC cells, hsa_circ_0077837 overexpression decreased PTEN expression, increased PTEN gene methylation, and reduced HCC827 cell apoptosis via PTEN. Overall, hsa_circ_0077837 is upregulated in NSCLC and downregulates PTEN by increasing its gene methylation to suppress cell apoptosis.List of abbreviations:Non-small cell lung cancer (NSCLC); circRNAs (circular RNAs); methylation-specific PCR (MSP).
环状 RNA (circRNA) hsa_circ_0077837 抑制结直肠癌。我们的研究研究了 hsa_circ_0077837 在非小细胞肺癌 (NSCLC) 中的参与情况。通过 RT-qPCR 研究了 64 例 NSCLC 患者的癌症和配对非癌组织中的 hsa_circ_0077837 和磷酸酶和张力蛋白同源物 (PTEN) 的表达。为了评估 hsa_circ_0077837 对 NSCLC 的预后价值，对这 64 例患者进行了 5 年的监测。通过 RT-qPCR 和 Western blot 确定了 hsa_circ_0077837 过表达的 NSCLC 细胞中 PTEN 的表达。通过甲基化特异性 PCR (MSP) 分析转染 hsa_circ_0077837 表达载体的细胞中 PTEN 基因的甲基化。通过细胞凋亡测定评估 hsa_circ_0077837 和 PTEN 在 NSCLC 细胞增殖中的作用。我们的数据显示，hsa_circ_0077837 在 NSCLC 中上调，并预测预后不良。此外，在 36 例晚期病例 (III 期和 IV 期) 中 hsa_circ_0077837 的表达水平高于 28 例早期病例 (I 期和 II 期)。hsa_circ_0077837 在癌组织中与 PTEN 呈负相关。在 NSCLC 细胞中，hsa_circ_0077837 过表达降低了 PTEN 表达，增加了 PTEN 基因甲基化，并通过 PTEN 降低了 HCC827 细胞凋亡。总的来说，hsa_circ_0077837 在 NSCLC 中上调，并通过增加其基因甲基化来抑制细胞凋亡从而下调 PTEN。缩写列表：非小细胞肺癌 (NSCLC)；circRNAs (环状 RNA)；甲基化特异性 PCR (MSP)。
