Division of Animal Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Sciences and Technology - Kashmir, SKUAST-K, Shuhama, Jammu, 190006, India.
Department of Biochemistry, School of Life Sciences Jaipur National University, Jaipur, India.
BMC Genomics. 2022 Mar 4;23(1):176. doi: 10.1186/s12864-022-08406-x.
Long noncoding RNAs (lncRNAs) are now proven as essential regulatory elements, playing diverse roles in many biological processes including mammary gland development. However, little is known about their roles in the bovine lactation process.
To identify and characterize the roles of lncRNAs in bovine lactation, high throughput RNA sequencing data from Jersey (high milk yield producer), and Kashmiri cattle (low milk yield producer) were utilized. Transcriptome data from three Kashmiri and three Jersey cattle throughout their lactation stages were utilized for differential expression analysis. At each stage (early, mid and late) three samples were taken from each breed. A total of 45 differentially expressed lncRNAs were identified between the three stages of lactation. The differentially expressed lncRNAs were found co-expressed with genes involved in the milk synthesis processes such as GPAM, LPL, and ABCG2 indicating their potential regulatory effects on milk quality genes. KEGG pathways analysis of potential cis and trans target genes of differentially expressed lncRNAs indicated that 27 and 48 pathways were significantly enriched between the three stages of lactation in Kashmiri and Jersey respectively, including mTOR signaling, PI3K-Akt signaling, and RAP1 signaling pathways. These pathways are known to play key roles in lactation biology and mammary gland development.
Expression profiles of lncRNAs across different lactation stages in Jersey and Kashmiri cattle provide a valuable resource for the study of the regulatory mechanisms involved in the lactation process as well as facilitate understanding of the role of lncRNAs in bovine lactation biology.
长链非编码 RNA（lncRNA）现已被证明是重要的调控元件，在包括乳腺发育在内的许多生物过程中发挥着多样化的作用。然而，它们在牛泌乳过程中的作用知之甚少。
为了鉴定和描述 lncRNA 在牛泌乳中的作用，利用了泽西牛（高乳产量生产者）和克什米尔牛（低乳产量生产者）的高通量 RNA 测序数据。利用来自三个克什米尔牛和三个泽西牛在泌乳阶段的转录组数据进行差异表达分析。每个品种在每个阶段（早期、中期和晚期）采集三个样本。共鉴定出 45 个在泌乳三个阶段中差异表达的 lncRNA。差异表达的 lncRNA 与参与乳合成过程的基因（如 GPAM、LPL 和 ABCG2）共表达，表明它们对乳质量基因具有潜在的调控作用。差异表达 lncRNA 的潜在顺式和反式靶基因的 KEGG 途径分析表明，在克什米尔和泽西牛的泌乳三个阶段之间，分别有 27 和 48 条途径显著富集，包括 mTOR 信号、PI3K-Akt 信号和 RAP1 信号途径。这些途径已知在泌乳生物学和乳腺发育中发挥关键作用。
泽西牛和克什米尔牛在不同泌乳阶段的 lncRNA 表达谱为研究泌乳过程中涉及的调控机制提供了有价值的资源，并有助于理解 lncRNA 在牛泌乳生物学中的作用。
