College of Veterinary Medicine, Gansu Agricultural University, Gansu, PR China.
College of Veterinary Medicine, Gansu Agricultural University, Gansu, PR China.
Biochem Biophys Res Commun. 2022 Apr 23;601:153-159. doi: 10.1016/j.bbrc.2022.02.061. Epub 2022 Feb 23.
Streptococcus agalactiae mastitis is one of the significant threats to the milk industry. The traditional antibiotic treatment method is easy to cause the emergence of resistant strains, and the problem of drug residue is increasingly severe. In this study, we designed and synthesized five lipopeptides. The antibacterial activity of different molecular structure lipopeptides against Streptococcus agalactiae was detected. Furthermore, the mouse mastitis model was established using Streptococcus agalactiae. The lipopeptides with better antibacterial effect were selected for the treatment experiment to evaluate the application value in the treatment of mastitis. The results showed that 4 of the synthesized lipopeptides had specific antibacterial activity. SLP3 and SLP4 have an excellent antibacterial effect and can treat murine mastitis caused by Streptococcus agalactiae infection within the safe concentration range. The results of this study can provide an excellent experimental basis for new antibiotics and clinical application in the treatment of dairy cow mastitis.
无乳链球菌乳腺炎是乳品业的重大威胁之一。传统的抗生素治疗方法容易导致耐药菌株的出现，药物残留问题日益严重。本研究设计并合成了五种脂肽。检测了不同分子结构脂肽对无乳链球菌的抗菌活性。此外，还建立了无乳链球菌诱导的小鼠乳腺炎模型。选择具有更好抗菌效果的脂肽进行治疗实验，以评估其在乳腺炎治疗中的应用价值。结果表明，合成的 4 种脂肽具有特定的抗菌活性。SLP3 和 SLP4 具有优异的抗菌效果，可在安全浓度范围内治疗无乳链球菌感染引起的小鼠乳腺炎。本研究结果可为新型抗生素的开发和临床应用于奶牛乳腺炎的治疗提供良好的实验基础。
