Gustafsson J A, Gustafsson S A, Nordenskjöld B, Okret S, Silfverswärd C, Wrange O
Cancer Res. 1978 Nov;38(11 Pt 2):4225-8.
Isoelectric focusing in polyacrylamide gel combined with limited proteolysis is a simple and specific method for quantitation of estradiol receptors in breast cancer tissue. At least eight different samples can be analyzed simultaneously on one gel, and the whole procedure, including sample preparation, takes less than 7 hr. In comparison with sucrose gradient centrifugation, isoelectric focusing is more sensitive, possibly due to the short time (1.5 to 2 hr) needed for the analysis. Furthermore, only one incubation with tritium-labeled estradiol is needed for an analysis, which means that a smaller amount of tumor tissue is needed than for most other methods. This fact allows analysis of the estrogen receptor content in tumor material obtained from fine-needle biopsy.
聚丙烯酰胺凝胶等电聚焦结合有限蛋白酶解是一种用于定量乳腺癌组织中雌二醇受体的简单且特异的方法。在一块凝胶上可同时分析至少八个不同样本，整个过程，包括样本制备，耗时不到7小时。与蔗糖梯度离心法相比，等电聚焦更灵敏，这可能是由于分析所需时间短（1.5至2小时）。此外，一次分析仅需用氚标记的雌二醇进行一次孵育，这意味着与大多数其他方法相比，所需的肿瘤组织量更少。这一事实使得能够对细针穿刺活检获得的肿瘤材料中的雌激素受体含量进行分析。
