Tobacco-associated with Methylophilus sp. FP-6 enhances phytoremediation of benzophenone-3 through regulating soil microbial community, increasing photosynthetic capacity and maintaining redox homeostasis of plant.

Benzophenone-3 (BP-3) has attracted widespread attention due to its large accumulation in the environment and its potential toxicity effects to human. This study aimed to investigate the effects of the combined application of tobacco and Methylophilus sp. strain FP-6 with both plant growth promoting (PGP) traits and BP-3 degradation function on BP-3 remediation in soil. The results showed that about 79.18% of BP-3 was removed from the soil after 30 days of plant culture inoculated with the FP-6 strain, which was significantly higher than the plant-alone treatment. Simultaneously, inoculation with strain FP-6 significantly improved growth performance, biomass production, antioxidant levels, osmoregulation substance, photosynthetic capacity and chlorophyll accumulation in tobacco. Moreover, the application of FP-6 shifted the bacterial community, and enhanced the abundance of BP-3-degrading or soil nutrient cycling-affecting bacteria (e.g., Chloroflexi, Bryobacter, MND1 and Myxococcales), which might be valuable for the promotion of plant growth and degradation of BP-3 in the soil. The results from this study gave first insights into the enhancement of BP-3 removal efficiency from soil by phytoremediation assisted with bacteria possessing both PGP properties and BP-3 degradation function. The role of soil bacterial community in this remediation process was also discussed.