Department of Cardiac Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India.
Department of Orthopaedics, All India Institute of Medical Sciences, New Delhi, 110029, India.
Eur J Nutr. 2022 Aug;61(5):2673-2685. doi: 10.1007/s00394-022-02844-9. Epub 2022 Mar 6.
The aim of the present study was to assess the effect of Bacillus coagulans Unique IS-2 supplementation on absorption and utilization of protein in resistance-trained males.
In this double blind, placebo-control trial, resistance-trained males (21.08 ± 2.84 years) were randomized to consume, either 20 g of whey protein powder {80% whey protein concentrate (WPC80), amounting to 15.4 g protein} with 2 billion CFU Bacillus coagulans Unique IS-2 (supplemental group) or 20 g of whey protein powder and lactose instead of Bacillus coagulans (placebo group) once daily for 60 days with a controlled resistance exercise protocol. The whey protein concentrate (WPC-80) given to both groups had a lactose content of 6.8%. Plasma-free amino acids (PFAAs) were determined at baseline, at 30 and 60 days of supplementation. Muscle strength, hypertrophy, VO2 max, and body composition, and other biochemical parameters were assessed at baseline and end line.
A positive effect of probiotic Bacillus coagulans Unique IS-2 supplementation was observed on protein absorption as evidenced by an increase in total PFAA by + 16.1% (p = 0.004). Branched chain amino acids (BCAA) comprising isoleucine (p = 0.016), leucine (p = 0.001), and valine (p = 0.002) were increased by + 33.1% in ITT analysis as compared to placebo after 60 days. At 30 days an increase in isoleucine by + 35% (p = 0.113), leucine by + 43% (p = 0.032), and valine by + 32% (p = 0.017) was observed in ITT analysis. Probiotic effect was shown on exercise performance as evidenced by an increase in one RM of leg press and vertical jump power by + 16.61% (p = 0.024) and + 7.86% (p = 0.007), respectively.
Significantly increased absorption of BCAA with supplementation of B. coagulans Unique IS-2 along with whey protein and improvement in leg press and vertical jump power was noted indicating the positive effect of the probiotic on muscle power in the lower body.
CTRI/2017/03/008117; Date:16.03.2017.
本研究旨在评估凝结芽孢杆菌独特株 IS-2 补充剂对阻力训练男性蛋白质吸收和利用的影响。
在这项双盲、安慰剂对照试验中，将 21.08±2.84 岁的阻力训练男性随机分为两组，分别摄入 20 克乳清蛋白粉[80%乳清蛋白浓缩物（WPC80），含 15.4 克蛋白质]，加 20 亿 CFU 凝结芽孢杆菌独特株 IS-2（补充组）或 20 克乳清蛋白粉和乳糖代替凝结芽孢杆菌（安慰剂组），每天一次，持续 60 天，并进行控制的阻力运动方案。两组给予的乳清蛋白浓缩物（WPC-80）的乳糖含量为 6.8%。在基线、30 天和 60 天补充时测定血浆游离氨基酸（PFAAs）。在基线和终线评估肌肉力量、肥大、最大摄氧量和身体成分以及其他生化参数。
补充益生菌凝结芽孢杆菌独特株 IS-2 对蛋白质吸收有积极影响，总 PFAA 增加了+16.1%（p=0.004）。支链氨基酸（BCAA）中的异亮氨酸（p=0.016）、亮氨酸（p=0.001）和缬氨酸（p=0.002）在 ITT 分析中分别增加了+33.1%，与安慰剂相比，在 60 天后。在 30 天时，异亮氨酸增加了+35%（p=0.113），亮氨酸增加了+43%（p=0.032），缬氨酸增加了+32%（p=0.017）。补充益生菌后，运动表现也有所改善，腿推和垂直跳跃力量的 1 RM 分别增加了+16.61%（p=0.024）和+7.86%（p=0.007）。
补充凝结芽孢杆菌独特株 IS-2 与乳清蛋白一起显著增加 BCAA 的吸收，并提高腿推和垂直跳跃力量，表明益生菌对下肢肌肉力量有积极影响。
CTRI/2017/03/008117；日期：2017 年 3 月 16 日。
