不同处理对大肠杆菌F11（P155）热不稳定肠毒素活性的影响。
Effect of different treatments on the activity of the heat labile enterotoxin of Escherichia coli F11(P155).
作者信息
Lallier R, Larivière S
出版信息
Can J Comp Med. 1978 Apr;42(2):214-8.
Abstract
A whole cell lysate preparation of the Escherichia coli strain F11(P155) was treated with the following agents: heat (60 degrees C for 30 minutes), pronase, lipase, amylase, formalin (0.1%), sodium lauryl sulfate (0.05%) and sarkosyl NL30 (0.05%). Except for the amylase treatment, the treated whole cell lysate was inactivated when tested in rabbit gut loops or on Chinese hamster ovary cells. The preparations treated with heat or formalin could be used to produce a neutralizing antiserum or could remove the neutralizing capacity of an anti-F11(P155) serum. Finally, the attempt to demonstrate enterotoxicity with the cytoplasmic membrane of the bacterial cell failed.
摘要
用以下试剂处理大肠杆菌菌株F11(P155)的全细胞裂解物制剂：加热（60℃，30分钟）、链霉蛋白酶、脂肪酶、淀粉酶、福尔马林（0.1%）、十二烷基硫酸钠（0.05%）和月桂酰肌氨酸钠NL30（0.05%）。除淀粉酶处理外，经处理的全细胞裂解物在兔肠袢或中国仓鼠卵巢细胞上进行测试时均失活。经加热或福尔马林处理的制剂可用于产生中和抗血清，或可消除抗F11(P155)血清的中和能力。最后，用细菌细胞质膜证明肠毒性的尝试失败了。
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