大肠杆菌鸡致死毒素的研究。
Studies on the chick-lethal toxin of Eecherichia coli.
作者信息
Truscott R B
出版信息
Can J Comp Med. 1973 Oct;37(4):375-81.
Abstract
A toxin which is lethal for two week old chicks has been recovered from strains of Escherichia coli O78:K80 of bovine and avian origin and from avian isolates of serogroups O2, O45 and O109. The toxin is heat-labile, antigenic, high in protein, inactivated by pronase, trypsin, amylase, and pancreatic lipase. The toxin may be precipitated by ammonium sulfate or TCA treatment from the supernatant obtained by repeated centrifugation of sonicated cells. Considerable purification has been obtained by column chromatography using Sepharose 6B.
摘要
从牛源和禽源的大肠杆菌O78:K80菌株以及血清群O2、O45和O109的禽源分离株中分离出一种对两周龄雏鸡具有致死性的毒素。该毒素对热不稳定、具有抗原性、蛋白质含量高，可被链霉蛋白酶、胰蛋白酶、淀粉酶和胰脂肪酶灭活。通过对超声处理后的细胞反复离心获得的上清液，经硫酸铵或三氯乙酸处理可使毒素沉淀。使用琼脂糖6B进行柱色谱法已实现了相当程度的纯化。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
The neurotoxin of Shigella shigae. I. Production, purification and properties of the toxin.
Br J Exp Pathol. 1953 Apr;34(2):202-16.
3
Cholera infection and toxin in the rabbit ileal loop.
J Infect Dis. 1966 Apr;116(2):183-90. doi: 10.1093/infdis/116.2.183.
4
Factors influencing in vitro skin permeability factor production by Vibrio cholerae.
J Bacteriol. 1969 Oct;100(1):27-34. doi: 10.1128/jb.100.1.27-34.1969.
5
The neurotoxins of gram-negative bacteria: the thermolabile endotoxin.
Ann N Y Acad Sci. 1966 Jun 30;133(2):685-99. doi: 10.1111/j.1749-6632.1966.tb52398.x.
6
Relation of structure to function in bacterial O-antigens. V. Nature of active sites in endotoxic lipopolysaccharides of Serratia marcescens.
Ann N Y Acad Sci. 1966 Jun 30;133(2):604-21. doi: 10.1111/j.1749-6632.1966.tb52392.x.
7
Biological studies on free endotoxin and a non-toxic material from culture supernatant fluids of Escherichia coli 078K80.
J Gen Microbiol. 1968 Mar;50(3):413-20. doi: 10.1099/00221287-50-3-413.
8
[Neutralization of E. coli neurotoxin. 1. Toxin neutralization in vitro].
Zentralbl Veterinarmed B. 1971 Oct;18(8):622-33.
9
The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.
J Med Microbiol. 1970 Aug;3(3):387-401. doi: 10.1099/00222615-3-3-387.
10
Cell wall composition and virulence in Escherichia coli.
J Exp Med. 1968 Sep 1;128(3):399-414. doi: 10.1084/jem.128.3.399.
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