Keyhani Jacqueline, Keyhani Ezzatollah, Attar Farnoosh, Haddadi Azam
Laboratory for Life Sciences, Saadat Abade, Sarve Sharghi 34, 19979, Tehran, Iran.
J Ind Microbiol Biotechnol. 2006 Mar;33(3):238-42. doi: 10.1007/s10295-005-0261-y. Epub 2005 Jun 30.
This research reports the sensitivity of a clinical isolate of Enterococcus faecalis to sodium N-lauroylsarcosinate (sarkosyl) and sodium dodecyl sulfate (SDS), as well as the efficiency of these detergents in curing the strain. Compared to Escherichia coli, Enterococcus faecalis was very sensitive to both detergents, with minimum inhibitory concentrations (MIC) for the latter being 100 times lower than for Escherichia coli. The clinical isolate of Enterococcus faecalis used in this study exhibited plasmid-borne resistance to kanamycin (MIC 2 mg/ml) and tetracycline (MIC 50 mug/ml); 3% curing was observed after growth in the presence of sarkosyl but no curing was observed after growth in the presence of either SDS or acridine orange. In contrast, 35% curing of plasmid-bearing Escherichia coli was observed after growth in the presence of either SDS or acridine orange, but none was observed after growth in the presence of sarkosyl.
本研究报告了粪肠球菌临床分离株对N-月桂酰肌氨酸钠（ Sarkosyl ）和十二烷基硫酸钠（ SDS ）的敏感性，以及这些去污剂治愈该菌株的效率。与大肠杆菌相比，粪肠球菌对这两种去污剂都非常敏感，后者的最小抑菌浓度（ MIC ）比大肠杆菌低100倍。本研究中使用的粪肠球菌临床分离株表现出对卡那霉素（ MIC 2 mg/ml ）和四环素（ MIC 50 μg/ml ）的质粒介导抗性；在Sarkosyl存在下生长后观察到3%的质粒消除，但在SDS或吖啶橙存在下生长后未观察到质粒消除。相比之下，在SDS或吖啶橙存在下生长后，观察到携带质粒的大肠杆菌有35%的质粒消除，但在Sarkosyl存在下生长后未观察到质粒消除。
