• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

霍乱弧菌毒力的生物化学 I. PF/霍乱肠毒素的纯化和生化特性。

Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin.

机构信息

Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.

出版信息

Infect Immun. 1970 Jun;1(6):546-54. doi: 10.1128/iai.1.6.546-554.1970.

DOI:10.1128/iai.1.6.546-554.1970
PMID:16557774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC415940/
Abstract

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Although not completely free from traces of vibriocidal antibody-stimulating activity, the resultant purified toxic immunogen appeared homogeneous as judged by immunoelectrophoresis, immunodiffusion, and disc electrophoresis. Advantages and disadvantages of this purification method are discussed in comparison with those of previously published techniques.

摘要

本文介绍了一种从培养上清液中浓缩、分离和纯化 PF/霍乱肠毒素的简单方法。用葡聚糖硫酸酯和硫酸铵沉淀，然后进行凝胶过滤和离子交换层析，得到高生物效价的 PF/肠毒素。尽管该方法不能完全去除杀菌抗体刺激活性的痕迹，但根据免疫电泳、免疫扩散和圆盘电泳的结果判断，所得纯化的毒性免疫原是均一的。本文还讨论了与先前发表的技术相比，该纯化方法的优缺点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a987/415940/467f51d872ab/iai00294-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a987/415940/23af41838ee6/iai00294-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a987/415940/467f51d872ab/iai00294-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a987/415940/23af41838ee6/iai00294-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a987/415940/467f51d872ab/iai00294-0040-b.jpg

相似文献

1
Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin.霍乱弧菌毒力的生物化学 I. PF/霍乱肠毒素的纯化和生化特性。
Infect Immun. 1970 Jun;1(6):546-54. doi: 10.1128/iai.1.6.546-554.1970.
2
Biochemistry of Vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis.霍乱弧菌毒力的生物化学：通过制备性圆盘电泳纯化霍乱肠毒素
Appl Environ Microbiol. 1976 Aug;32(2):288-93. doi: 10.1128/aem.32.2.288-293.1976.
3
Purification and some properties of a non-o1 Vibrio cholerae enterotoxin that is identical to cholera enterotoxin.一种与霍乱肠毒素相同的非O1群霍乱弧菌肠毒素的纯化及其某些特性
Infect Immun. 1983 Mar;39(3):1128-35. doi: 10.1128/iai.39.3.1128-1135.1983.
4
Evidence that a non-O1 Vibrio cholerae produces enterotoxin that is similar but not identical to cholera enterotoxin.有证据表明，非O1群霍乱弧菌产生的肠毒素与霍乱肠毒素相似但并不相同。
Infect Immun. 1983 Sep;41(3):896-901. doi: 10.1128/iai.41.3.896-901.1983.
5
Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment.从环境中分离出的一株非O1群霍乱弧菌产生霍乱样肠毒素。
Infect Immun. 1981 Oct;34(1):90-7. doi: 10.1128/iai.34.1.90-97.1981.
6
Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid.实验性霍乱的发病机制。霍乱毒素和类霍乱毒素的制备与分离。
J Exp Med. 1969 Jul 1;130(1):185-202. doi: 10.1084/jem.130.1.185.
7
Development of a purified cholera toxoid. I. Purification of toxin.纯化霍乱类毒素的研制。I. 毒素的纯化。
Infect Immun. 1974 Feb;9(2):294-303. doi: 10.1128/iai.9.2.294-303.1974.
8
The application of QAE-Sephadex for the purification of two staphylococcal enterotoxins. I. Purification of enterotoxin C2.QAE-葡聚糖在两种葡萄球菌肠毒素纯化中的应用。I. 肠毒素C2的纯化。
Biochim Biophys Acta. 1975 May 30;393(1):148-58. doi: 10.1016/0005-2795(75)90225-1.
9
Biochemistry of vibrio cholerae virulence. II. Skin permeability factor-cholera enterotoxin production in a chemically defined medium.霍乱弧菌毒力的生物化学。II. 在化学成分确定的培养基中皮肤渗透因子-霍乱肠毒素的产生。
Infect Immun. 1971 Nov;4(5):611-8. doi: 10.1128/iai.4.5.611-618.1971.
10
Partial purification and properties of somatic antigen spontaneously released from Vibrio cholerae.霍乱弧菌自发释放的菌体抗原的部分纯化及特性
Infect Immun. 1975 Jul;12(1):187-92. doi: 10.1128/iai.12.1.187-192.1975.

引用本文的文献

1
Biochemistry of vibrio cholerae virulence. II. Skin permeability factor-cholera enterotoxin production in a chemically defined medium.霍乱弧菌毒力的生物化学。II. 在化学成分确定的培养基中皮肤渗透因子-霍乱肠毒素的产生。
Infect Immun. 1971 Nov;4(5):611-8. doi: 10.1128/iai.4.5.611-618.1971.
2
Biochemistry of Vibrio cholerae virulence. 3. Nutritional requirements for toxin production and the effects of pH on toxin elaboration in chemically defined media.霍乱弧菌毒力的生物化学。3. 毒素产生的营养需求以及pH值对化学限定培养基中毒素合成的影响。
Infect Immun. 1973 Apr;7(4):567-72. doi: 10.1128/iai.7.4.567-572.1973.
3
Experimental studies on cholera immunization. II. Evidence for protective antitoxic immunity mediated by serum antibodies as well as local antibodies.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera.在霍乱粪便和培养滤液中发现的一种通透因子（毒素）及其被霍乱恢复期血清中和的情况。
Nature. 1965 Aug 7;207(997):614-6. doi: 10.1038/207614a0.
3
Isolation of cholera toxins by dextran sulfate precipitation.通过硫酸葡聚糖沉淀法分离霍乱毒素。
霍乱免疫的实验研究。II. 血清抗体和局部抗体介导保护性抗毒素免疫的证据。
Infect Immun. 1972 May;5(5):662-7. doi: 10.1128/iai.5.5.662-667.1972.
4
Vibrio cholerae enterotoxin and its mode of action.霍乱弧菌肠毒素及其作用方式。
Bacteriol Rev. 1971 Mar;35(1):1-13. doi: 10.1128/br.35.1.1-13.1971.
5
Experiments with cholera toxin detoxified with glutaraldehyde.用戊二醛解毒的霍乱毒素实验。
Bull World Health Organ. 1974;51(6):633-9.
6
Development of a purified cholera toxoid. I. Purification of toxin.纯化霍乱类毒素的研制。I. 毒素的纯化。
Infect Immun. 1974 Feb;9(2):294-303. doi: 10.1128/iai.9.2.294-303.1974.
7
Biochemistry of Vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis.霍乱弧菌毒力的生物化学：通过制备性圆盘电泳纯化霍乱肠毒素
Appl Environ Microbiol. 1976 Aug;32(2):288-93. doi: 10.1128/aem.32.2.288-293.1976.
8
Effect of different treatments on the activity of the heat labile enterotoxin of Escherichia coli F11(P155).不同处理对大肠杆菌F11（P155）热不稳定肠毒素活性的影响。
Can J Comp Med. 1978 Apr;42(2):214-8.
J Bacteriol. 1968 Oct;96(4):1443-5. doi: 10.1128/jb.96.4.1443-1445.1968.
4
In vitro production of choleragen and vascular permeability factor by Vibrio cholerae.霍乱弧菌体外产生霍乱毒素和血管通透性因子。
J Bacteriol. 1968 Jul;96(1):126-30. doi: 10.1128/jb.96.1.126-130.1968.
5
Factors influencing in vitro skin permeability factor production by Vibrio cholerae.影响霍乱弧菌体外皮肤渗透因子产生的因素。
J Bacteriol. 1969 Oct;100(1):27-34. doi: 10.1128/jb.100.1.27-34.1969.
6
Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid.实验性霍乱的发病机制。霍乱毒素和类霍乱毒素的制备与分离。
J Exp Med. 1969 Jul 1;130(1):185-202. doi: 10.1084/jem.130.1.185.
7
Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin.霍乱毒素：2型回肠袢反应性毒素的纯化及初步特性分析
J Bacteriol. 1968 Oct;96(4):1137-43. doi: 10.1128/jb.96.4.1137-1143.1968.