Garg Bhavuk, Malhotra Rajesh, Mittal Samarth, Kumar Arvind, Mehta Nishank, Malik Garima, Gupta Manish, Trikha Vivek
Department of Orthopaedics, All India Institute of Medical Sciences, New Delhi, India.
Department of Orthopaedics, Jai Prakash Narayan Apex Trauma Centre, New Delhi, India.
Indian J Orthop. 2021 Nov 13;56(3):399-411. doi: 10.1007/s43465-021-00561-9. eCollection 2022 Mar.
The expression pattern of micro-RNAs (miRNA) has been implicated in the pathomechanism of various bone disorders, and has a role in differentiation of osteoblasts and osteoclasts. The purpose of the study was to investigate the differential miRNA profiles of osteoporotic hip fractures compared to young patients with hip fractures.
Blood samples from ten osteoporosis patients and ten young, healthy patients, presenting with acute hip fractures were collected and subjected to an initial miRNA profiling to detect those miRNAs with significant variations between the two groups based on polymerase chain reactions performed in duplicate. A real-time quantitative polymerase chain reaction-based analysis was then performed for validation of specific miRNAs that were significantly different between the two groups.
A total of 182 miRNAs were analyzed. Thirty-nine of them showed significant differences between the two groups in the initial miRNA profiling. The validation results suggested that five miRNAs related to bone metabolism had significantly different expression among the osteoporotic hip fracture group compared to the young, healthy group: miR-23b-3p and miR-140-3p were up-regulated; miR-21-5p, miR-122-5p and miR-125b-5p were down-regulated.
Differential expression of selected miRNAs in patients with osteoporotic hip fracture suggests a possible role of miRNAs as potential biomarkers in prevention or timely prediction of osteoporotic fractures in the elderly. Further research is required to elucidate the mechanism of their involvement in osteoporosis.
Not applicable.
微小RNA（miRNA）的表达模式与多种骨骼疾病的发病机制有关，并且在成骨细胞和破骨细胞的分化中发挥作用。本研究的目的是调查骨质疏松性髋部骨折患者与年轻髋部骨折患者之间miRNA谱的差异。
收集10例骨质疏松患者和10例年轻健康的急性髋部骨折患者的血样，进行初步的miRNA谱分析，以基于重复进行的聚合酶链反应检测两组之间有显著差异的miRNA。然后进行基于实时定量聚合酶链反应的分析，以验证两组之间有显著差异的特定miRNA。
共分析了182种miRNA。其中39种在初步的miRNA谱分析中显示两组之间有显著差异。验证结果表明，与骨代谢相关的5种miRNA在骨质疏松性髋部骨折组与年轻健康组之间的表达有显著差异：miR-23b-3p和miR-140-3p上调；miR-21-5p、miR-122-5p和miR-125b-5p下调。
骨质疏松性髋部骨折患者中选定miRNA的差异表达表明，miRNA作为潜在生物标志物在预防或及时预测老年人骨质疏松性骨折中可能发挥作用。需要进一步研究以阐明它们参与骨质疏松症的机制。
不适用。
