宏基因组下一代测序技术诊断并发现病因的脑脓肿：病例报告
Brain Abscess Caused by and Diagnosed by Metagenomic Next-Generation Sequencing: A Case Report.
作者信息
Yang Jianhua, Xie Shuhua, Li Junda, Xia Han, Liu Xianghong
机构信息
Department of Neurology, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, China.
Department of Scientific Affairs, Hugobiotech Co., Ltd., Beijing, China.
出版信息
Front Med (Lausanne). 2022 Feb 16;9:803554. doi: 10.3389/fmed.2022.803554. eCollection 2022.
BACKGROUND
Brain abscesses caused by are rare and difficult to diagnose. Conventional methods for diagnosing Nocardia species include blood culture, microscopy, and tissue slice, but the performance is not satisfied. We report a case of brain abscess due to diagnosed by metagenomic next-generation sequencing (mNGS).
CASE PRESENTATION
We report a case of a 58-year-old man with brain abscess caused by . The patient had a history of pemphigus and required long-term methylprednisolone administration. No pathogen was detected in blood culture, cerebrospinal fluid (CSF) culture, and fast-acid staining. mNGS identified in the CSF. The symptoms and signs of the patient were significantly improved after changing the antibiotics accordingly to sensitive antibiotics.
CONCLUSION
Metagenomic next-generation sequencing (mNGS) is helpful for early diagnosis and subsequent treatment of -associated meningitis and encephalitis, avoiding brain surgery. Early and accurate diagnosis and prompt antibiotic treatment reduced its mortality.
背景
由[未提及具体病因]引起的脑脓肿罕见且难以诊断。诊断诺卡菌属的传统方法包括血培养、显微镜检查和组织切片，但效果并不理想。我们报告一例通过宏基因组下一代测序（mNGS）诊断出的由[未提及具体病因]引起的脑脓肿病例。
病例介绍
我们报告一例58岁男性由[未提及具体病因]引起脑脓肿的病例。该患者有天疱疮病史，需要长期使用甲基泼尼松龙。血培养、脑脊液（CSF）培养和抗酸染色均未检测到病原体。mNGS在脑脊液中鉴定出[未提及具体病因]。根据药敏结果更换抗生素后，患者的症状和体征明显改善。
结论
宏基因组下一代测序（mNGS）有助于早期诊断及后续治疗与[未提及具体病因]相关的脑膜炎和脑炎，避免脑部手术。早期准确诊断和及时的抗生素治疗降低了其死亡率。
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