Diagnosis of Meningitis with Metagenomic Next-Generation Sequencing: A Case Report.

BACKGROUND

meningitis is a rare postoperative complication of neurosurgery. Accurate and early diagnosis of remains challenging because of the limitations of traditional detection methods. Metagenomic next-generation sequencing (mNGS) is an advanced technique with high sensitivity and specificity for identifying infectious pathogens; however, its application in diagnosing meningitis has not been widely studied.

CASE PRESENTATION

We report the case of a 61-year-old man who presented with fever and headache after neurosurgical treatment for a cerebral hemorrhage. Empiric antibiotic therapy was ineffective. Traditional culture of pathogens and serological testing yielded negative results, but was detected in the cerebrospinal fluid by mNGS. After further verification by polymerase chain reaction (PCR), the patient's clinical treatment was adjusted accordingly. With targeted antibiotic intervention, the patient's symptoms were effectively alleviated, and clinical indicators returned to normal levels. Furthermore, the abundance of decreased significantly compared to the initial mNGS reading after targeted treatment, indicating that the infection caused by was effectively controlled.

CONCLUSION

Using mNGS, we found that may be a candidate causative agent of meningitis. The technique also has the advantage of timeliness and accuracy that traditional cultures cannot achieve. A combination of mNGS with PCR is recommended to identify pathogens in the early stages of infectious diseases to administer targeted clinical medication.