Rozing J, Brons N H, van Ewijk W, Benner R
Cell Tissue Res. 1978 May 18;189(1):19-30. doi: 10.1007/BF00223118.
The recovery of the B lymphocyte compartments was investigated in lethally irradiated mice reconstituted with fetal liver cells. This was done by means of immunofluorescence on frozen sections of spleen, lymph nodes and Peyer's patches. The first B lymphocyte recovery in the spleen was observed on day 8, a few days earlier than in lymph nodes and Peyer's patches (day 13). These early B cells in the spleen were found in the central part of the periarteriolar lymphatic sheath (PALS). Later on, while increasing in number, the B cells formed growing follicles at the periphery of the PALS. Subsequently, brightly fluorescent B cells appeared in the marginal zone, which surrounded the follicles. Another two weeks later, around day 30, also germinal center formation was observed in the follicles of the spleen. B cell development in lymph nodes and Peyer's patches started somewhat later than in the spleen, but once started, the recovery of the different compartments was completed very fast. Germinal center reactions were found in lymph nodes and Peyer's patches already on day 25, and thus earlier than in the spleen, but later than the first occurrence of the strongly fluorescent cells in the marginal zone. Apparently, germinalcenter formation is not essential for the recovery of the population of brightly fluorescent B cells in the marginal zone after irradiation and reconstitution.
在接受致死剂量照射并用胎肝细胞重建的小鼠中，研究了B淋巴细胞区室的恢复情况。这是通过对脾脏、淋巴结和派伊尔结的冰冻切片进行免疫荧光来完成的。脾脏中首次观察到B淋巴细胞恢复是在第8天，比淋巴结和派伊尔结（第13天）早几天。脾脏中的这些早期B细胞位于动脉周围淋巴鞘（PALS）的中央部分。后来，B细胞数量增加，在PALS周边形成不断增大的滤泡。随后，明亮荧光的B细胞出现在围绕滤泡的边缘区。又过了两周左右，在第30天左右，脾脏滤泡中也观察到生发中心的形成。淋巴结和派伊尔结中的B细胞发育比脾脏稍晚开始，但一旦开始，不同区室的恢复非常迅速。在第25天就已在淋巴结和派伊尔结中发现生发中心反应，因此比脾脏早，但比边缘区首次出现强荧光细胞晚。显然，生发中心的形成对于照射和重建后边缘区明亮荧光B细胞群体的恢复并非必不可少。
