居间序列的加工：一种不能从前体tRNA中切除居间序列的新酵母突变体。

Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.

作者信息

Hopper A K, Schultz L D, Shapiro R A

出版信息

Cell. 1980 Mar;19(3):741-51. doi: 10.1016/s0092-8674(80)80050-x.

Abstract

By using conditional loss of suppression an an assay, we have been successful in screening for a yeast mutant which is defective in tRNA processing. The los1-1 mutation causes an accumulation of a subset of precursor tRNAs at the nonpermissive temperature. These pre-tRNAs are like those which accumulate in the yeast mutant ts 136 (rna1) in that they have transcribed intervening sequences. The mutations at los1-1 and rna1 complement and segregate independently of each other. The los1-1 mutation affects the expression of all 8 tyrosine-inserting suppressor loci, but does not seem to affect rRNA or mRNA synthesis.

摘要

通过使用条件性抑制缺失作为一种检测方法，我们成功筛选到了一个在tRNA加工过程中存在缺陷的酵母突变体。los1-1突变导致在非允许温度下前体tRNA的一个子集积累。这些前体tRNA与在酵母突变体ts136（rna1）中积累的那些相似，因为它们具有转录的间隔序列。los1-1和rna1处的突变相互互补且独立分离。los1-1突变影响所有8个酪氨酸插入抑制位点的表达，但似乎不影响rRNA或mRNA的合成。