Nakano K, Abe S, Sohmura Y
Int J Immunopharmacol. 1986;8(3):347-55. doi: 10.1016/0192-0561(86)90117-7.
Cytotoxic activity of recombinant human TNF (rHu-TNF) on various human cell lines was examined in vitro. rHu-TNF exerted a cytostatic effect on various types of human tumor cells such as carcinoma, sarcoma, leukemia, melanoma and other types. When the cytocidal effect was examined on the tumor cells which were cytostatically susceptible to rHu-TNF, the cytocidal effect of rHu-TNF was also noticed on many of these tumor cells. However, some tumor cells were affected cytostatically only. Human diploid cells were not affected cytostatically or cytocidally by rHu-TNF. WI-38 VA13 cells which are an SV-40-transformed derivative of WI-38 diploid cells, were affected both cytostatically and cytocidally by rHu-TNF. These results suggest that rHu-TNF exerts cytostatic and cytocidal effects against a broad spectrum of human tumor cells, and its cytotoxic activity is tumor-specific.
在体外检测了重组人肿瘤坏死因子（rHu-TNF）对多种人类细胞系的细胞毒性活性。rHu-TNF对多种类型的人类肿瘤细胞，如癌、肉瘤、白血病、黑色素瘤及其他类型肿瘤细胞发挥了细胞生长抑制作用。当检测rHu-TNF对细胞生长抑制敏感的肿瘤细胞的杀伤作用时，也发现rHu-TNF对许多此类肿瘤细胞具有杀伤作用。然而，一些肿瘤细胞仅受到细胞生长抑制的影响。人二倍体细胞未受到rHu-TNF的细胞生长抑制或杀伤作用的影响。WI-38 VA13细胞是WI-38二倍体细胞的SV-40转化衍生物，受到rHu-TNF的细胞生长抑制和杀伤作用的影响。这些结果表明，rHu-TNF对广泛的人类肿瘤细胞发挥细胞生长抑制和杀伤作用，其细胞毒性活性具有肿瘤特异性。
