Farzollahi Masomeh, Tayefi-Nasrabadi Hamid, Mohammadnejad Daryosh, Abedelahi Ali
Departments of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Obstet Gynaecol Res. 2016 May;42(5):526-35. doi: 10.1111/jog.12933. Epub 2016 Feb 2.
The aim of this study was to evaluate the effects of preventive vitamin E (α-tocopherol) on antral follicle development and embryogenesis of oocytes obtained after vitrification of mouse ovarian tissue.
Female Balb/c mice were killed by cervical dislocation after the injection of pregnant mare's serum gonadotrophin (10 IU) and their ovaries were randomly divided into three groups: control or non-vitrified (n = 10), vitrification 1 (5, 10% ethylene glycol + 5, 10% dimethylsulfoxide) (n = 15), and vitrification 2 (10, 15% ethylene glycol + 10, 15% dimethylsulfoxide) (n = 15) with ascending concentration of cryoprotectants. After toxicity tests and vitrification-warming, mechanically isolated antral follicles were cultured in α-minimum essential medium, which was supplemented with or without α-tocopherol (100 μM). The follicular maturation rates and embryo development were collected and assessed. Also, the viability, morphology and ultrastructure of derived antral follicles from vitrified ovaries were analyzed.
The morphology and ultrastructure of follicles were well preserved in the vitrified groups and α-tocopherol supplementation of culture media significantly increased the proportion of oocytes that reached metaphase II blastocyst rates compared to non-α-tocopherol supplemented media (P < 0.01).
Vitamin E improves in vitro maturation rates and blastocyst rates of oocytes that are isolated from vitrified ovarian tissue.
本研究旨在评估预防性使用维生素E（α-生育酚）对小鼠卵巢组织玻璃化冷冻后获得的窦卵泡发育及卵母细胞胚胎发生的影响。
给雌性Balb/c小鼠注射孕马血清促性腺激素（10 IU）后，通过颈椎脱臼法处死小鼠，将其卵巢随机分为三组：对照组或非玻璃化组（n = 10）、玻璃化1组（5%、10%乙二醇 + 5%、10%二甲基亚砜）（n = 15）、玻璃化2组（10%、15%乙二醇 + 10%、15%二甲基亚砜）（n = 15），冷冻保护剂浓度依次升高。经过毒性测试及玻璃化-复温处理后，将机械分离的窦卵泡在添加或不添加α-生育酚（100 μM）的α-最低必需培养基中培养。收集并评估卵泡成熟率及胚胎发育情况。此外，还对玻璃化卵巢来源的窦卵泡的活力、形态及超微结构进行了分析。
玻璃化组卵泡的形态及超微结构保存良好，与未添加α-生育酚的培养基相比，添加α-生育酚的培养基显著提高了达到中期II胚泡率的卵母细胞比例（P < 0.01）。
维生素E可提高从玻璃化冷冻的卵巢组织中分离出的卵母细胞的体外成熟率及胚泡率。
