远距离病毒诊断中免疫荧光法与免疫过氧化物酶法的比较：一项世界卫生组织协作研究
Comparison of immunofluorescence and immunoperoxidase methods for viral diagnosis at a distance: a WHO collaborative study.
作者信息
Gardner P S, Grandien M, McQuillin J
出版信息
Bull World Health Organ. 1978;56(1):105-10.
Nasopharyngeal secretions collected in Newcastle were examined in both Newcastle and Stockholm for the presence of influenza virus type A and respiratory syncytial (RS) virus by immunofluorescence and immunoperoxidase techniques. A total of 139 specimens were examined in this way and the agreement between the two centres for immunofluorescence was 94% for influenza virus A, 95% for respiratory syncytial virus, and 95% for negative specimens. This technique can therefore be used for examining specimens taken at great distances from the collecting laboratory. The results of the immunoperoxidase technique were unsatisfactory mainly because of the presence of endogenous peroxidase. Measures taken to remove this also destroyed RS virus antigen. It is premature to introduce the immunoperoxidase technique for rapid virus diagnosis, but in due course, when the problems outlined in this article are solved, it could become a useful technique.
在纽卡斯尔采集的鼻咽分泌物样本，在纽卡斯尔和斯德哥尔摩两地，运用免疫荧光法和免疫过氧化物酶技术对甲型流感病毒及呼吸道合胞体（RS）病毒进行检测。总共139份样本通过这种方式进行了检测，两个中心在免疫荧光检测方面，对甲型流感病毒的检测结果一致性为94%，对呼吸道合胞体病毒为95%，对阴性样本为95%。因此，这项技术可用于检测从采集实验室远距离获取的样本。免疫过氧化物酶技术的检测结果并不理想，主要原因是存在内源性过氧化物酶。为去除该物质所采取的措施也破坏了呼吸道合胞体病毒抗原。将免疫过氧化物酶技术用于快速病毒诊断为时尚早，但在适当的时候，当本文所述问题得到解决后，它可能会成为一项有用的技术。
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