Cevenini R, Donati M, Moroni A, Franchi L, Rumpianesi F
J Clin Microbiol. 1983 Oct;18(4):947-9. doi: 10.1128/jcm.18.4.947-949.1983.
Samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (IPA). The IPA was compared with the immunofluorescence assay and with cell culture isolation. Respiratory syncytial virus antigen-positive cells were detected by both IPA and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. The agreement between virus isolation and IPA and IFA was 89%. The applicability of IPA to rapid viral diagnosis of respiratory syncytial virus infection is discussed.
采用免疫过氧化物酶测定法（IPA）对70例患急性呼吸道疾病的婴幼儿的鼻咽分泌物样本进行检测，以确定是否存在呼吸道合胞病毒。将IPA与免疫荧光测定法以及细胞培养分离法进行比较。在28份标本中，通过IPA和免疫荧光测定法均检测到呼吸道合胞病毒抗原阳性细胞；25份样本在细胞培养中呈阳性。病毒分离与IPA和免疫荧光测定法之间的一致性为89%。本文讨论了IPA在呼吸道合胞病毒感染快速病毒诊断中的适用性。
