一种荧光探针水解隔离等温 PCR 的开发，用于快速灵敏的现场检测非洲猪瘟病毒。
Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus.
机构信息
Beijing Wantai Biopharmaceutical Co., Ltd, Beijing, 102206, China.
Luoyang Putai Bio-Tech Co. Ltd, Luoyang, 471003, China.
出版信息
Virol Sin. 2022 Jun;37(3):462-464. doi: 10.1016/j.virs.2022.03.002. Epub 2022 Mar 4.
Abstract
• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.
摘要
• 建立了一种基于探针的隔热等温 PCR（iiPCR）检测方法，用于快速和现场检测 ASF。• 开发的 iiPCR 与 OIE 推荐的实时 PCR 具有相似的灵敏度和特异性。• 在 iiPCR 中，无需提取 DNA，即可直接将血液样本用作 PCR 模板。
Zotero 插件