扩增子分类器:一种基于序列相似性的无参考扩增子分类工具以及用于构建共有序列的工具。

Amplicon_sorter: A tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences.

作者信息

Vierstraete Andy R, Braeckman Bart P

机构信息

Laboratory of aging physiology and Molecular Evolution University of Gent Gent Belgium.

出版信息

Ecol Evol. 2022 Mar 1;12(3):e8603. doi: 10.1002/ece3.8603. eCollection 2022 Feb.

Abstract

Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology excels at long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.

摘要

牛津纳米孔技术公司(ONT)的第三代测序技术,凭借其便携性和低成本测序的可能性,在生态研究中越来越受欢迎。尽管该技术在长读长测序方面表现出色,但它也可应用于扩增子测序。ONT的缺点是原始读数质量较低。因此,生成高质量的一致性序列仍然是一项挑战。我们展示了Amplicon_sorter,这是一种基于ONT测序扩增子的序列和长度相似性进行无参考排序并构建可靠一致性序列的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd24/8888255/218e8e6c0b57/ECE3-12-e8603-g002.jpg

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