[Binding properties of insulin receptors in adipose tissue and hepatic plasma membranes in rats during fasting and in alloxan diabetes].

The affinity and number of receptors were determined using Scatchard's, Meytes' and Rose's graphic analyses in the hepatic and fat plasmatic membranes of rats in various duration of hunger (2 and 7 days) and alloxan diabetes (3 and 7 days after alloxan administration) to test a hypothesis that increased affinity of insulin receptors was the first phase of their activation preceding an increase in the number of insulin binding sites. Irrespective of hunger duration an increase in the number and affinity of receptors was detected in the hepatic membranes and an increase in their affinity in the fat membranes. In diabetes of various duration the number of receptors in the membranes of both tissues increased. With an increase in the period of exposure the degree of expression of receptor changes decreased in the fat but not in the hepatic membranes.