Griep A E, DeLuca H F
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5539-43. doi: 10.1073/pnas.83.15.5539.
Retinoic acid-induced differentiation of mouse F9 teratocarcinoma cells is known to be accompanied by changes in gene expression. We examined the expression of the c-myc gene during retinoic acid-induced differentiation of F9 cells. Retinoic acid caused a 50% reduction in the level of c-myc mRNA after 3 hr of treatment and a 90% reduction after 12 hr. We have also examined several features of c-myc genomic structure in F9 cells, PYS2 (differentiated parietal yolk sac) cells, and liver--namely, methylation, amplification, and gross organization. Comparison of Hpa II and Msp I digests of DNAs from F9, PYS2, and liver showed that the c-myc gene in F9 cells is hypomethylated relative to that in PYS2 cells and in liver. The Hpa II sites that undergo methylation during differentiation were found to be in the second intron. Gross structural changes of the c-myc gene have not occurred in F9 or PYS2, and the c-myc gene is not amplified.
已知维甲酸诱导的小鼠F9畸胎瘤细胞分化伴随着基因表达的变化。我们检测了F9细胞在维甲酸诱导分化过程中c-myc基因的表达。维甲酸处理3小时后，c-myc mRNA水平降低了50%，处理12小时后降低了90%。我们还检测了F9细胞、PYS2（分化的壁层卵黄囊）细胞和肝脏中c-myc基因的基因组结构的几个特征，即甲基化、扩增和总体组织情况。对来自F9、PYS2和肝脏的DNA进行Hpa II和Msp I酶切分析比较，结果显示F9细胞中的c-myc基因相对于PYS2细胞和肝脏中的该基因处于低甲基化状态。发现在分化过程中发生甲基化的Hpa II位点位于第二个内含子中。F9或PYS2细胞中c-myc基因未发生总体结构变化，且该基因未扩增。
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