大肠杆菌的亚精胺合酶：speE基因的定位
Spermidine synthase of Escherichia coli: localization of the speE gene.
作者信息
Tabor C W, Tabor H, Xie Q W
出版信息
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6040-4. doi: 10.1073/pnas.83.16.6040.
Abstract
We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was cloned into pBR322. Deletion of 105 bases upstream of speE caused a coordinate loss of both activities, indicating that speE and speD constitute a single operon. speE and speD have also been cloned separately in a high-expression vector; strains carrying these plasmids overproduce the respective enzymes.
摘要
我们已获得缺乏亚精胺合酶（腐胺氨基丙基转移酶）的大肠杆菌突变体，并发现突变基因（speE）位于编码S-腺苷甲硫氨酸脱羧酶（speD）的基因紧邻上游；这些基因位于大肠杆菌染色体的2.7分钟处。这两个基因存在于克隆到pBR322中的一段1795个碱基对的大肠杆菌DNA片段中。speE上游105个碱基的缺失导致两种活性协同丧失，表明speE和speD构成一个单一操纵子。speE和speD也已分别克隆到一个高表达载体中；携带这些质粒的菌株过量产生各自的酶。
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本文引用的文献
1
A note on the use of cellulose phosphate cation-exchange paper for the separation of catecholamines, and some other biogenic amines.关于使用磷酸纤维素阳离子交换纸分离儿茶酚胺及其他一些生物胺的说明。
J Pharm Pharmacol. 1962 Nov;14:746-9. doi: 10.1111/j.2042-7158.1962.tb11170.x.
2
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
3
S-adenosylmethionine decarboxylase of Escherichia coli. Studies on the covalently linked pyruvate required for activity.大肠杆菌的S-腺苷甲硫氨酸脱羧酶。对活性所需的共价连接丙酮酸的研究。
J Biol Chem. 1982 Oct 25;257(20):12063-8.
4
Expression of the cloned genes encoding the putrescine biosynthetic enzymes and methionine adenosyltransferase of Escherichia coli (speA, speB, speC and metK).编码大肠杆菌腐胺生物合成酶和甲硫氨酸腺苷转移酶的克隆基因（speA、speB、speC和metK）的表达。
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5
Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.用克隆在质粒上的无活性基因替换大肠杆菌的fip基因。
J Bacteriol. 1984 Sep;159(3):1034-9. doi: 10.1128/jb.159.3.1034-1039.1984.
6
Cloning of the Escherichia coli genes for the biosynthetic enzymes for polyamines.大肠杆菌多胺生物合成酶基因的克隆。
Methods Enzymol. 1983;94:117-21. doi: 10.1016/s0076-6879(83)94019-3.
7
An integrated and simplified approach to cloning into plasmids and single-stranded phages.一种整合且简化的克隆到质粒和单链噬菌体中的方法。
Methods Enzymol. 1983;101:78-89. doi: 10.1016/0076-6879(83)01006-x.
8
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
9
Polyamines.多胺
Annu Rev Biochem. 1984;53:749-90. doi: 10.1146/annurev.bi.53.070184.003533.
10
Spermidine biosynthesis. Purification and properties of propylamine transferase from Escherichia coli.亚精胺生物合成。大肠杆菌丙胺转移酶的纯化及性质
J Biol Chem. 1973 Apr 10;248(7):2480-6.
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