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环状 RNA_ANKIB1 通过结合 microRNA-26b-5p 并调节增强子结合蛋白 2 来加速骨肉瘤的化疗耐药性。

Circular RNA_ANKIB1 accelerates chemo-resistance of osteosarcoma via binding microRNA-26b-5p and modulating enhancer of zeste homolog 2.

机构信息

Department Orthopedics, Huai'an Second People's Hospital, Huai'an City, JiangSu Province, China.

Department Orthopedics, Huaian Hospital Affiliated to Xuzhou Medical University, Huai'an City, Jiangsu, China.

出版信息

Bioengineered. 2022 Mar;13(3):7351-7366. doi: 10.1080/21655979.2022.2037869.

DOI:10.1080/21655979.2022.2037869
PMID:35264070
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8974058/
Abstract

Osteosarcoma is a common bone malignancy in children and adolescents. Chemotherapeutic drug resistance is the major factor impacting the surgical outcome and prognosis of patients with osteosarcoma. This investigation assessed the role and mechanism of circular RNA_ANKIB1 in the development of osteosarcoma. The circular RNA (circ) _ANKIB1, microRNA (miR)-26b-5p, enhancer of zeste homolog 2 (EZH2) expression in OS samples was investigated through RT-qPCR. The EZH2, multidrug resistance protein 1 (MRP1), P-gp, and lipoprotein receptor-related protein (LRP) protein expressions were analyzed through western blot. The association between circ_ANKIB1 and the occurrence of clinic-pathological features in OS patients was assessed; the circular features of circ_ANKIB1 were analyzed. The hFOB1.19, KHOS, U2-OS OS cells were used to study the semi-inhibitory concentration IC50 of Doxorubicin (DXR)-resistant cells, clone formation, invasion, and apoptosis. The luciferase assay was used to study the binding of circ-ANKIB1 with miR-26b-5p and the targeting of miR-26b-5p with EZH2. In experiments were performed via subcutaneous tumorigenic experiments. MiR-26b-5p in OS tissues and cells and DXR-resistant OS tissues and cells was silenced while circ_ANKIB1 and EZH2 were elevated. Circ_ANKIB1 silencing elevated miR-26b-5p, repressed EZH2, MRP1, P-gp, LRP, IC50, and elevated OS advancement. Circ_ANKIB1 bind miR-26b-5p. Reduced miR-26b-5p revered the influence of silencing circ_ANKIB1 on DXR resistant OS cells. MiR-26b-5p targeted EZH2, and EZH2 elevation reversed the impact of increasing miR-26b-5p on DXR resistant cells. Circ_ANKIB1 silencing suppressed DXR-resistant OS cells in . In conclusion, Circ_ANKIB1 binds miR-26b-5p and modulates EZH2 to accelerate the chemo-resistance of osteosarcoma.

摘要

骨肉瘤是儿童和青少年中常见的骨恶性肿瘤。化疗药物耐药性是影响骨肉瘤患者手术效果和预后的主要因素。本研究评估了环状 RNA _ANKIB1 在骨肉瘤发生发展中的作用和机制。通过 RT-qPCR 检测 OS 样本中环状 RNA（circ）_ANKIB1、微小 RNA（miR）-26b-5p、增强子的组蛋白 H2A 家族成员 EZH2 的表达。通过 Western blot 分析多药耐药蛋白 1（MRP1）、P-糖蛋白（P-gp）和脂蛋白受体相关蛋白（LRP）的蛋白表达。评估 circ_ANKIB1 与骨肉瘤患者临床病理特征发生的相关性；分析 circ_ANKIB1 的环状特征。使用 hFOB1.19、KHOS、U2-OS 骨肉瘤细胞研究多柔比星（Doxorubicin，DXR）耐药细胞的半抑制浓度 IC50、克隆形成、侵袭和凋亡。通过荧光素酶报告基因实验研究 circ_ANKIB1 与 miR-26b-5p 的结合以及 miR-26b-5p 与 EZH2 的靶向作用。进行了皮下致瘤实验。沉默骨肉瘤组织和细胞及 DXR 耐药骨肉瘤组织和细胞中的 miR-26b-5p，同时上调 circ_ANKIB1 和 EZH2。沉默 circ_ANKIB1 上调 miR-26b-5p，抑制 EZH2、MRP1、P-gp、LRP、IC50，并促进骨肉瘤进展。Circ_ANKIB1 与 miR-26b-5p 结合。降低 miR-26b-5p 逆转了沉默 circ_ANKIB1 对 DXR 耐药 OS 细胞的影响。miR-26b-5p 靶向 EZH2，上调 miR-26b-5p 逆转了增加 miR-26b-5p 对 DXR 耐药细胞的影响。沉默 circ_ANKIB1 抑制了裸鼠体内的 DXR 耐药骨肉瘤细胞。总之，Circ_ANKIB1 结合 miR-26b-5p 并调节 EZH2 以加速骨肉瘤的化疗耐药性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/8aa7100b5203/KBIE_A_2037869_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/693cf2a2eb98/KBIE_A_2037869_UF0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/f3eed0377418/KBIE_A_2037869_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/8624b7e6dc8e/KBIE_A_2037869_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/abbaa82e8b44/KBIE_A_2037869_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/49b7d87cc356/KBIE_A_2037869_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/6aadab25048b/KBIE_A_2037869_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/abf04eac8dfd/KBIE_A_2037869_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/8aa7100b5203/KBIE_A_2037869_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/693cf2a2eb98/KBIE_A_2037869_UF0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/f3eed0377418/KBIE_A_2037869_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/8624b7e6dc8e/KBIE_A_2037869_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/abbaa82e8b44/KBIE_A_2037869_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/49b7d87cc356/KBIE_A_2037869_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/6aadab25048b/KBIE_A_2037869_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/abf04eac8dfd/KBIE_A_2037869_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/787b/8974058/8aa7100b5203/KBIE_A_2037869_F0007_OC.jpg

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本文引用的文献

1
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J Oncol. 2021 Oct 21;2021:3408937. doi: 10.1155/2021/3408937. eCollection 2021.
2
Circ_0001721 enhances doxorubicin resistance and promotes tumorigenesis in osteosarcoma through miR-758/TCF4 axis.Circ_0001721通过miR-758/TCF4轴增强骨肉瘤对多柔比星的耐药性并促进肿瘤发生。
Cancer Cell Int. 2021 Jul 2;21(1):336. doi: 10.1186/s12935-021-02016-5.
3
CircPRDM2 Contributes to Doxorubicin Resistance of Osteosarcoma by Elevating EZH2 via Sponging miR-760.
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Cell Commun Signal. 2024 Nov 29;22(1):572. doi: 10.1186/s12964-024-01952-9.
4
Screening of a Prognostic Gene Signature for Relapsed/Refractory Acute Myeloid Leukemia Based on Altered Circulating CircRNA Profiles.基于循环circRNA谱改变筛选复发/难治性急性髓系白血病的预后基因特征
Int J Gen Med. 2024 Jul 8;17:2967-2979. doi: 10.2147/IJGM.S466364. eCollection 2024.
5
Metal-organic framework-mediated siRNA delivery and sonodynamic therapy for precisely triggering ferroptosis and augmenting ICD in osteosarcoma.金属有机框架介导的siRNA递送与声动力疗法精确触发骨肉瘤铁死亡并增强免疫原性细胞死亡
Mater Today Bio. 2024 Apr 10;26:101053. doi: 10.1016/j.mtbio.2024.101053. eCollection 2024 Jun.
6
Competitive endogenous network of circRNA, lncRNA, and miRNA in osteosarcoma chemoresistance.环状 RNA、长链非编码 RNA 和 microRNA 在骨肉瘤化疗耐药中的竞争性内源性网络。
Eur J Med Res. 2023 Sep 16;28(1):354. doi: 10.1186/s40001-023-01309-x.
7
MAT2A inhibits the ferroptosis in osteosarcoma progression regulated by miR-26b-5p.MAT2A抑制由miR-26b-5p调控的骨肉瘤进展中的铁死亡。
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4
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5
Osteosarcoma: a review of current and future therapeutic approaches.骨肉瘤：当前和未来治疗方法的综述。
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Noncoding RNAs in osteosarcoma: Implications for drug resistance.非编码 RNA 在骨肉瘤中的作用：与耐药性的关系。
Cancer Lett. 2021 Apr 28;504:91-103. doi: 10.1016/j.canlet.2021.02.007. Epub 2021 Feb 12.
7
Circular RNA circANKIB1 promotes the progression of osteosarcoma by regulating miR-217/PAX3 axis.环状RNA circANKIB1通过调控miR-217/PAX3轴促进骨肉瘤进展。
J Bone Oncol. 2021 Jan 14;27:100347. doi: 10.1016/j.jbo.2021.100347. eCollection 2021 Apr.
8
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Cancer Biother Radiopharm. 2023 Nov;38(9):585-595. doi: 10.1089/cbr.2020.4240. Epub 2020 Dec 22.
9
Overexpression of miR-329-3p sensitizes osteosarcoma cells to cisplatin through suppression of glucose metabolism by targeting LDHA.miR-329-3p 的过表达通过靶向 LDHA 抑制葡萄糖代谢使骨肉瘤细胞对顺铂敏感。
Cell Biol Int. 2021 Apr;45(4):766-774. doi: 10.1002/cbin.11476. Epub 2021 Feb 10.
10
RAB31 is targeted by miR-26b and serves a role in the promotion of osteosarcoma.RAB31是miR-26b的靶标，在骨肉瘤进展中发挥作用。
Oncol Lett. 2020 Nov;20(5):244. doi: 10.3892/ol.2020.12106. Epub 2020 Sep 15.