Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Borg El-Arab, Alexandria, 21934, Egypt.
Department of Marine Environment, National Institute of Oceanography and Fisheries, NIOF, Egypt.
Sci Rep. 2022 Mar 9;12(1):3841. doi: 10.1038/s41598-022-06600-1.
The purpose of this study was to test the nematicidal activity of extracts of two marine algae (Colpomenia sinuosa and Corallina mediterranea) and their synthesized silver nanoparticles against root-knot nematodes (Meloidogyne incognita) that infest tomato plants. Scanning electron microscopy (SEM) revealed that nanoparticles had aggregated into anisotropic Ag particles, and transmission electron microscopy (TEM) revealed that the particle sizes were less than 40 nm. Fourier Transform Infrared Spectroscopy (FT-IR) analysis revealed that the obtained nanoparticles had a sharp absorbance between 440 and 4000 cm, with 13 distinct peaks ranging from 474 to 3915 cm. Methylene chloride extracts and nanoparticles synthesized from both algae species were used to treat M. incognita. C. sinuosa nanoparticles had the highest nematicidal activity of any treatment. Furthermore, and in contrast to other treatments, C. sinuosa nanoparticles reduced the number of nematode galls, egg-masses per root, and eggs/egg mass, while also improving plant growth parameters. C. sinuosa's methylene chloride extract was more active than C. mediterranea's, and the most effective eluent of this solvent was hexane: methylene chloride: ethyl acetate (1: 0.5: 0.5, v/v/v). When applied to M. incognita, the third fraction of this eluent was the most effective, resulting in 87.5% mortality after 12 h and 100% mortality after 24 and 72 h of exposure. The presence of seven bioactive constituents was discovered during the analysis of this fraction. In conclusion, the silver nanoparticles synthesized from C. sinuosa could be used as alternative chemical nematicides.
本研究旨在测试两种海洋藻类（皱边石莼和地中海红皮藻）及其合成银纳米粒子对寄生番茄植物的根结线虫（南方根结线虫）的杀线虫活性。扫描电子显微镜（SEM）显示，纳米颗粒已聚集成长方体状的各向异性 Ag 颗粒，而透射电子显微镜（TEM）显示，颗粒尺寸小于 40nm。傅里叶变换红外光谱（FT-IR）分析表明，所获得的纳米颗粒在 440 至 4000cm 之间具有尖锐的吸收峰，有 13 个明显的峰分布在 474 至 3915cm 之间。两种藻类合成的二氯甲烷提取物和纳米颗粒均用于处理南方根结线虫。皱边石莼纳米颗粒的杀线虫活性最高。此外，与其他处理方式相比，皱边石莼纳米颗粒减少了线虫根结、每根根的卵块和卵/卵块的数量，同时也改善了植物生长参数。皱边石莼的二氯甲烷提取物比地中海红皮藻更活跃，而这种溶剂的最有效洗脱液是正己烷：二氯甲烷：乙酸乙酯（1:0.5:0.5，v/v/v）。当应用于南方根结线虫时，这种洗脱液的第三馏分效果最佳，暴露 12 小时后死亡率为 87.5%，暴露 24 小时和 72 小时后死亡率均为 100%。在分析该馏分时发现了七种具有生物活性的成分。综上所述，皱边石莼合成的银纳米颗粒可作为替代化学杀线虫剂使用。
