College of Life Science, Jilin Agricultural University, Changchun, 130118, China.
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, 130117, China.
Sci Rep. 2022 Mar 9;12(1):3876. doi: 10.1038/s41598-022-07787-z.
Daily rhythms are found in almost all organisms, and they comprise one of the most basic characteristics of living things. Daily rhythms are generated and mainly regulated by circadian clock. Bats have attracted interest from researchers because of their unique biological characteristics. However, little is known about the molecular underpinnings of daily rhythms in bats. In this study, we used RNA-Seq to uncover the daily rhythms of gene expression in the brains of Asian particolored bats over the 24-h day. Accordingly, four collected time points corresponding to four biological states, rest, sleep, wakefulness, and active, were selected. Several groups of genes with different expression levels in these four states were obtained suggested that different physiological processes were active at various biological states, including drug metabolism, signaling pathways, and the circadian rhythm. Furthermore, downstream analysis of all differentially expressed genes in these four states suggested that groups of genes showed daily rhythms in the bat brain. Especially for Per1, an important circadian clock gene was identified with rhythmic expression in the brain of Asian particolored bat. In summary, our study provides an overview of the brain transcriptomic differences in different physiological states over a 24-h cycle.
昼夜节律几乎存在于所有生物体中，是生物的最基本特征之一。昼夜节律由生物钟产生并主要进行调控。由于蝙蝠具有独特的生物学特征，因此引起了研究人员的关注。然而，关于蝙蝠昼夜节律的分子基础知之甚少。在这项研究中，我们使用 RNA-Seq 技术揭示了亚洲双色蝙蝠大脑中基因表达的昼夜节律，持续 24 小时。相应地，选择了四个采集时间点，对应于休息、睡眠、清醒和活跃四种生物状态。在这四种状态下，获得了几组表达水平不同的基因，这表明不同的生理过程在不同的生物状态下是活跃的，包括药物代谢、信号通路和昼夜节律。此外，对这四种状态下所有差异表达基因的下游分析表明，蝙蝠大脑中的几组基因表现出昼夜节律。特别是，重要的生物钟基因 Per1 在亚洲双色蝙蝠的大脑中被鉴定出具有节律性表达。总之，我们的研究提供了一个在 24 小时周期内不同生理状态下大脑转录组差异的概述。
