Gharib Amal F, Eed Emad M, Khalifa Amany S, Raafat Nermin, Shehab-Eldeen Somaia, Alwakeel Hany R, Darwiesh Ehab, Essa Abdallah
Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif, 21944, Saudi Arabia.
Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt.
Int J Gen Med. 2022 Mar 3;15:2427-2436. doi: 10.2147/IJGM.S354842. eCollection 2022.
Circulatory microRNAs (miRNAs) have the potential to be employed as markers for cancer detection and as prognostic tools for disease management. As a result, our goal was to explore the effectiveness of serum miRNA-96-5p and miRNA-99a-5p as diagnostic tools in hepatocellular carcinoma (HCC).
Blood samples were collected from 55 patients with HCV-induced HCC, 55 patients with HCV-induced liver cirrhosis, and 55 healthy controls. The expression levels of miRNA-96-5p and miRNA-99a-5p were measured using quantitative RT-PCR.
miRNA-96-5p expression levels were increased in HCC patient sera, while miRNA-99a-5p levels were reduced. According to ROC curve analysis, using a combination of circulating miRNA-96-5p, miRNA-99a-5-, and alpha-fetoprotein (AFP) improves the accuracy of diagnoses for HCC, with an area under the curve (AUC) of 0.97, compared to AUCs of 0.82, 0.86, and 0.73, respectively, for the individual biomarkers. Furthermore, the present data suggested that higher serum miRNA-96-5p levels were linked to larger tumors and metastasis, whereas lower serum miRNA-99a-5p levels were exclusively linked to HCC metastasis.
Using miRNA-96-5p and miRNA-99a-5p in combination with AFP increased both sensitivity and specificity for the diagnosis of HCC. Furthermore, serum levels were linked to tumor size and metastasis. These findings suggested that serum miRNA-96-5p and miRNA-99a-5p could be used as non-invasive biomarkers for the diagnosis of HCC.
循环微小RNA（miRNA）有潜力用作癌症检测的标志物以及疾病管理的预后工具。因此，我们的目标是探讨血清miRNA - 96 - 5p和miRNA - 99a - 5p作为肝细胞癌（HCC）诊断工具的有效性。
收集了55例丙型肝炎病毒（HCV）诱发的HCC患者、55例HCV诱发的肝硬化患者以及55例健康对照者的血液样本。采用定量逆转录聚合酶链反应（RT - PCR）检测miRNA - 96 - 5p和miRNA - 99a - 5p的表达水平。
HCC患者血清中miRNA - 96 - 5p表达水平升高，而miRNA - 99a - 5p水平降低。根据受试者工作特征（ROC）曲线分析，联合循环miRNA - 96 - 5p、miRNA - 99a - 5p和甲胎蛋白（AFP）可提高HCC诊断的准确性，曲线下面积（AUC）为0.97，而单个生物标志物的AUC分别为0.82、0.86和0.73。此外，目前的数据表明，血清miRNA - 96 - 5p水平较高与肿瘤较大及转移有关，而血清miRNA - 99a - 5p水平较低仅与HCC转移有关。
miRNA - 96 - 5p和miRNA - 99a - 5p联合AFP可提高HCC诊断的敏感性和特异性。此外，血清水平与肿瘤大小和转移有关。这些发现提示血清miRNA - 96 - 5p和miRNA - 99a - 5p可作为HCC诊断的非侵入性生物标志物。
