Department of Nuclear Medicine, Jingzhou Central Hospital, Jingzhou, Hubei 434020, China.
Department of Thyroid Vascular Surgery, Jingzhou Central Hospital, Jingzhou, Hubei 434020, China.
Comput Math Methods Med. 2022 Feb 28;2022:3585626. doi: 10.1155/2022/3585626. eCollection 2022.
Thyroid cancer (TC) is one of the widely diagnosed carcinomas in women before the age of 30. Nevertheless, there is currently a lack of specific biomarkers for predicting the prognosis of TC. Long noncoding RNAs (lncRNAs) were important regulators in human cancer progression as previously described. Unfortunately, there is little known on these lncRNAs' functions and molecular mechanisms in TC. In our literature, we found that LOC554202 (MIR31HG) was upregulated in TC samples and correlated with clinicopathological features, including M stage, N stage, and lymph nodes examined status in TC. In addition, we found that LOC554202 overexpression was evidently correlated with high immune infiltrate levels of CD8+ T cells, macrophage, neutrophil, myeloid dendritic cells, and B cells in TC. Knockdown of LOC554202 impeded TC cell proliferation and cycle progression. We found that LOC554202 had an association with metabolic pathways, vesicle-mediated transport, tricarboxylic acid cycle, Hedgehog signaling pathway, and Hippo signaling pathway in TC. Reducing LOC554202 hindered TC cell proliferation and cycle progression. Finally, we found that LOC554202 participated in modulating the expression of the regulators of Hippo signaling and TCA pathway, such as CCND2, CCND3, SDHC, SDHD, SUCLA2, and SUCLG1. We thought that this study would largely enhance our understanding of LOC554202's functional roles in human TC progression and immune response.
甲状腺癌 (TC) 是 30 岁以下女性中广泛诊断出的癌种之一。然而，目前缺乏预测 TC 预后的特异性生物标志物。长链非编码 RNA (lncRNA) 如前所述，是人类癌症进展中的重要调控因子。不幸的是，目前对这些 lncRNA 在 TC 中的功能和分子机制知之甚少。在我们的文献中，我们发现 LOC554202（MIR31HG）在 TC 样本中上调，并与包括 TC 中的 M 期、N 期和淋巴结检查状态在内的临床病理特征相关。此外，我们发现 LOC554202 的过表达与 TC 中 CD8+T 细胞、巨噬细胞、中性粒细胞、髓样树突状细胞和 B 细胞的高免疫浸润水平明显相关。下调 LOC554202 抑制 TC 细胞增殖和周期进展。我们发现 LOC554202 在 TC 中与代谢途径、囊泡介导的运输、三羧酸循环、Hedgehog 信号通路和 Hippo 信号通路有关。减少 LOC554202 会阻碍 TC 细胞的增殖和周期进展。最后，我们发现 LOC554202 参与调节 Hippo 信号通路和 TCA 通路的调节剂的表达，如 CCND2、CCND3、SDHC、SDHD、SUCLA2 和 SUCLG1。我们认为这项研究将大大提高我们对 LOC554202 在人类 TC 进展和免疫反应中的功能作用的理解。
