Kotanagi H, Takahashi T, Masuko T, Hashimoto Y, Koyama K
Tohoku J Exp Med. 1986 Apr;148(4):353-60. doi: 10.1620/tjem.148.353.
A monoclonal antibody was prepared by hybridizing mouse myeloma cells with spleen cells from the mouse which was immunized with human colon cancer transplanted in nude mice. The reactivity of the monoclonal antibody, named A7, was tested by immunoperoxidase method. A7 reacted strongly with human adenocarcinoma cell lines and carcinoembryonic antigen (CEA). In surgical specimens, A7 reacted with 10 cancer tissues and 2 normal colon mucosa from 19 colorectal cancer patients. A7 did not react with other cancers. It was thought that A7 reacted with colon- or colon cancer-specific CEA. The reactivity of A7 with colorectal cancers was markedly reduced by preoperative irradiation.
通过将小鼠骨髓瘤细胞与用移植于裸鼠的人结肠癌免疫的小鼠脾细胞杂交，制备了一种单克隆抗体。用免疫过氧化物酶法检测了名为A7的单克隆抗体的反应性。A7与人腺癌细胞系和癌胚抗原（CEA）反应强烈。在手术标本中，A7与19例结直肠癌患者的10个癌组织和2个正常结肠黏膜反应。A7与其他癌症无反应。认为A7与结肠或结肠癌特异性CEA反应。术前放疗显著降低了A7与结直肠癌的反应性。
