Cell Biology Program, Research Institute Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Division of Gastroenterology, Hepatology and Nutrition, Department of Paediatrics, University of Toronto, Toronto, ON M5S 1A1, Canada.
Nutrients. 2022 Feb 28;14(5):1014. doi: 10.3390/nu14051014.
Human milk oligosaccharides are complex, non-digestible carbohydrates that directly interact with intestinal epithelial cells to alter barrier function and host inflammation. Oligosaccharide composition varies widely between individual mothers, but it is unclear if this inter-individual variation has any impact on intestinal epithelial barrier function and gut inflammation.
Human milk oligosaccharides were extracted from the mature human milk of four individual donors. Using an in vitro model of intestinal injury, the effects of the oligosaccharides on the intestinal epithelial barrier and select innate and adaptive immune functions were assessed.
Individual oligosaccharide compositions shared comparable effects on increasing transepithelial electrical resistance and reducing the macromolecular permeability of polarized (Caco-2Bbe1) monolayers but exerted distinct effects on the localization of the intercellular tight junction protein zona occludins-1 in response to injury induced by a human enteric bacterial pathogen , serotype O157:H7. Immunoblots showed the differential effects of oligosaccharide compositions in reducing host chemokine interleukin 8 expression and inhibiting of p38 MAP kinase activation.
These results provide evidence of both shared and distinct effects on the host intestinal epithelial function that are attributable to inter-individual differences in the composition of human milk oligosaccharides.
人乳低聚糖是复杂的、不可消化的碳水化合物，它们直接与肠上皮细胞相互作用，改变屏障功能和宿主炎症。寡糖组成在个体母亲之间差异很大，但尚不清楚这种个体间的差异是否对肠上皮屏障功能和肠道炎症有任何影响。
从四位个体供体的成熟人乳中提取人乳低聚糖。使用肠损伤的体外模型，评估了低聚糖对肠上皮屏障和选择性先天和适应性免疫功能的影响。
个体低聚糖组成在增加跨上皮电阻和减少极化（Caco-2Bbe1）单层的大分子通透性方面具有相似的作用，但在人肠道病原体、血清型 O157:H7 诱导的损伤后，对细胞间紧密连接蛋白 zonula occludens-1 的定位有不同的作用。免疫印迹显示低聚糖组成在减少宿主趋化因子白细胞介素 8 的表达和抑制 p38 MAP 激酶激活方面具有不同的作用。
这些结果提供了证据，证明人乳低聚糖组成的个体差异与人乳低聚糖对宿主肠上皮功能既有共同作用，也有独特作用。
