The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
Science and Technology Service Center of Lin'an, Hangzhou 311300, China.
Molecules. 2022 Feb 27;27(5):1581. doi: 10.3390/molecules27051581.
is able to secrete laccase isoenzymes including constitutive and inducible forms, and has potential application for bioremediation of environmental pollutants. Here, an inducible group B laccase from MX2 was heterologously expressed in , and its yield reached 2.59 U/mL after 5 days of methanol inducing culture. The optimal pH and temperature of recombinant laccase (rLac1) to 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were 2.5 and 60 °C, respectively. Metal ions showed different effect on rLac1 which Mg, Cu, and K increased enzyme activity as their concentration increased, whereas Zn, Na, and Fe inhibited enzyme activity as their concentration increased. rLac1 showed good tolerance to organic solvents, and more than 42% of its initial activity remained in 10% organic solvents. Additionally, rLac1 exhibited a more efficient decolorization ability for remazol brilliant blue R (RBBR) than for acid red 1 (AR1), crystal violet (CV), and neutral red (NR). Molecular docking results showed RBBR has a stronger binding affinity with laccase than other dyes by interacting with substrate binding cavity of enzyme. The results indicated rLac1 may be a potential candidate for dye removal from textile wastewater.
能够分泌漆酶同工酶，包括组成型和诱导型，具有环境污染物生物修复的潜在应用。在这里，从 MX2 中异源表达了诱导型 B 组漆酶，经过 5 天甲醇诱导培养，其产量达到 2.59 U/mL。重组漆酶（rLac1）对 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的最适 pH 和温度分别为 2.5 和 60°C。金属离子对 rLac1 表现出不同的影响，随着浓度的增加，Mg、Cu 和 K 增加了酶的活性，而 Zn、Na 和 Fe 则随着浓度的增加抑制了酶的活性。rLac1 对有机溶剂具有良好的耐受性，在 10%有机溶剂中仍保留超过 42%的初始活性。此外，rLac1 对媒介蓝 R（RBBR）的脱色能力比酸性红 1（AR1）、结晶紫（CV）和中性红（NR）更强。分子对接结果表明，RBBR 通过与酶的底物结合腔相互作用，与其他染料相比，与漆酶具有更强的结合亲和力。结果表明，rLac1 可能是从纺织废水中去除染料的潜在候选物。
