Li Yuanjun, Wang Yanan, Huang Longyu, Chen Chunrong, An Na, Zheng Xiaoke
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Institute of Cotton Research, Chinese Academy of Agricultural Sciences (CAAS), Anyang 455000, China.
Molecules. 2022 Mar 1;27(5):1634. doi: 10.3390/molecules27051634.
is an important medicinal plant that has long been used in Chinese traditional medicine. Acteoside, one of the bioactive components from , possessed various pharmacological activities for human health; however, the molecular mechanism of acteoside formation is not fully understood. In the current study, a novel tyrosine decarboxylase (designated as RgTyDC2) was identified from the transcriptome. Biochemical analysis of RgTyDC2 showed RgTyDC2 uses tyrosine and dopa as the substrate to produce tyramine and dopamine, respectively, and it displays higher catalytic efficiency toward tyrosine than dopa. Moreover, the transcript level of was consistent with the accumulation pattern of acteoside in , supporting its possible role in the biosynthesis of acteoside in vivo.
是一种重要的药用植物，长期以来一直用于中国传统医学。松果菊苷是该植物的生物活性成分之一，对人体健康具有多种药理活性；然而，松果菊苷形成的分子机制尚未完全清楚。在本研究中，从该植物的转录组中鉴定出一种新型酪氨酸脱羧酶（命名为RgTyDC2）。对RgTyDC2的生化分析表明，RgTyDC2分别以酪氨酸和多巴为底物产生酪胺和多巴胺，并且它对酪氨酸的催化效率高于多巴。此外，该植物的转录水平与松果菊苷的积累模式一致，支持其在体内松果菊苷生物合成中的可能作用。
