Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.
Division of Bio-Medical Science & Technology, KIST School, University of Science and Technology, Seoul 02792, Korea.
Molecules. 2022 Mar 2;27(5):1649. doi: 10.3390/molecules27051649.
has been consumed as a food and as a traditional medicine for treating autoimmune diseases and aging in diverse countries. A previous study showed that a mixture of and prohibited adipocyte differentiation and lipid accumulation in preadipocytes and suppressed diet-induced obesity. Nevertheless, the mechanism of to regulate energy homeostasis solely through thermogenic signaling remains unclear. Thus, we investigated its effects on energy homeostasis using fed to C57BL/6 mice with a 45% high-fat diet. Chronic consumption of decreased the body weight of obese mice with increasing food intakes and improved metabolic-syndrome-related phenotypes. Therefore, we further tested its thermogenic effects. Cold chamber experiments and qPCR studies indicated that elevated thermogenic signaling pathways, demonstrated by increased body temperature and uncoupling protein 1 (UCP1) signaling in the white and brown adipose tissues. Afzelin is one major known compound derived from . Hence, the isolated compound afzelin was treated with preadipocytes and brown adipocytes for cell viability and luciferase assay, respectively, to further examine its thermogenic effect. The studies showed that the response of afzelin was responsible for cell viability and the increased UCP1. In conclusion, our data indicated that elevated peripheral thermogenic signaling through increased UCP1 via afzelin activation and ameliorated diet-induced obesity.
在不同国家，[成分名称] 一直被作为食物和传统药物用于治疗自身免疫性疾病和衰老。先前的一项研究表明，[成分名称] 和[成分名称] 的混合物可阻止前体脂肪细胞的脂肪分化和脂质积累，并抑制饮食诱导的肥胖。然而，[成分名称] 通过产热信号单独调节能量平衡的机制尚不清楚。因此，我们使用高脂肪饮食喂养 C57BL/6 小鼠来研究其对能量平衡的影响。[成分名称] 的慢性消耗增加了肥胖小鼠的食物摄入量，降低了它们的体重，并改善了与代谢综合征相关的表型。因此，我们进一步测试了其产热作用。冷室实验和 qPCR 研究表明，[成分名称] 通过增加体温和白色及棕色脂肪组织中的解偶联蛋白 1（UCP1）信号，增强了产热信号通路。[成分名称] 是一种主要的已知化合物，来源于[成分名称]。因此，我们用分离得到的化合物[成分名称] 分别处理前体脂肪细胞和棕色脂肪细胞，进行细胞活力和荧光素酶测定，以进一步研究其产热作用。研究表明，[成分名称] 通过激活 UCP1 来增加细胞活力和 UCP1 的表达，从而对产热产生反应。综上所述，我们的数据表明，[成分名称] 通过激活 afzelin 增加 UCP1 来增强外周产热信号，从而改善饮食诱导的肥胖。
