Yang Shuangjia, Zheng Le, Chen Zilong, Jiao Zeren, Liu Tianqing, Nie Yi, Kang Yue, Pan Bo, Song Kedong
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
Artie McFerrin Department of Chemical Engineering, College Station, Texas A&M University, Texas, TX 77843-3122, USA.
Materials (Basel). 2022 Mar 4;15(5):1935. doi: 10.3390/ma15051935.
In spite of many anti-cancer drugs utilized in clinical treatment, cancer is still one of the diseases with the highest morbidity and mortality worldwide, owing to the complexity and heterogeneity of the tumor microenvironment. Compared with conventional 2D tumor models, 3D scaffolds could provide structures and a microenvironment which stimulate native tumor tissues more accurately. The extracellular matrix (ECM) is the main component of the cell in the microenvironment that is mainly composed of three-dimensional nanofibers, which can form nanoscale fiber networks, while the decellularized extracellular matrix (dECM) has been widely applied to engineered scaffolds. In this study, pig kidney was used as the source material to prepare dECM scaffolds. A chemical crosslinking method was used to improve the mechanical properties and other physical characteristics of the decellularized pig kidney-derived scaffold. Furthermore, a human breast cancer cell line (MCF-7) was used to further investigate the biocompatibility of the scaffold to fabricate a tumor model. The results showed that the existence of nanostructures in the scaffold plays an important role in cell adhesion, proliferation, and differentiation. Therefore, the pig kidney-derived matrix scaffold prepared by decellularization could provide more cell attachment sites, which is conducive to cell adhesion and proliferation, physiological activities, and tumor model construction.
尽管临床治疗中使用了多种抗癌药物，但由于肿瘤微环境的复杂性和异质性，癌症仍然是全球发病率和死亡率最高的疾病之一。与传统的二维肿瘤模型相比，三维支架可以提供更准确模拟天然肿瘤组织的结构和微环境。细胞外基质（ECM）是微环境中细胞的主要组成部分，主要由三维纳米纤维组成，可形成纳米级纤维网络，而去细胞化细胞外基质（dECM）已广泛应用于工程支架。在本研究中，以猪肾为原料制备dECM支架。采用化学交联方法改善去细胞猪肾衍生支架的力学性能和其他物理特性。此外，使用人乳腺癌细胞系（MCF-7）进一步研究该支架的生物相容性，以构建肿瘤模型。结果表明，支架中纳米结构的存在对细胞黏附、增殖和分化起着重要作用。因此，通过去细胞化制备的猪肾衍生基质支架可以提供更多的细胞附着位点，有利于细胞黏附、增殖、生理活动和肿瘤模型构建。
