Asensio-Regalado Carlos, Alonso-Salces Rosa María, Gallo Blanca, Berrueta Luis A, Era Benedetta, Pintus Francesca, Caddeo Carla
Department of Analytical Chemistry, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, Spain.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CIAS-IIPROSAM, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, Mar de Plata 7600, Argentina.
Nanomaterials (Basel). 2022 Feb 23;12(5):746. doi: 10.3390/nano12050746.
Polyphenols are gaining increasing interest due to their beneficial properties to human health. Grape pomace, the by-product of wine production, is a source of these bioactive compounds. An extract from grape pomace was obtained and characterized qualitatively and quantitatively. The major components found were anthocyanins, flavan-3-ols, and flavonols. To improve the bioavailability of these compounds, the extract was formulated in phospholipid vesicles, namely transfersomes. Spherical unilamellar vesicles around 100 nm each were obtained. The antioxidant activity of both the extract and the transfersomes was evaluated by using colorimetric assays (i.e., DPPH, FRAP, and Folin-Ciocalteu). The cells' viability and the antioxidant activity were assessed in keratinocytes. The results showed that the extract and the transfersomes had no cytotoxic effects and exerted remarkable antioxidant activity, which was more evident in a vesicle formulation. These findings highlighted the potential of the grape pomace extract and the efficacy of the incorporation into phospholipid vesicles.
由于多酚类物质对人体健康有益，它们越来越受到关注。葡萄皮渣是葡萄酒生产的副产品，是这些生物活性化合物的一个来源。制备了葡萄皮渣提取物，并对其进行了定性和定量表征。发现的主要成分是花青素、黄烷-3-醇和黄酮醇。为了提高这些化合物的生物利用度，将提取物制成磷脂囊泡，即传递体。获得了平均直径约100 nm的球形单层囊泡。通过比色法（即DPPH、FRAP和福林-西奥尔特法）评估了提取物和传递体的抗氧化活性。在角质形成细胞中评估了细胞活力和抗氧化活性。结果表明，提取物和传递体没有细胞毒性作用，并具有显著的抗氧化活性，在囊泡制剂中更为明显。这些发现突出了葡萄皮渣提取物的潜力以及将其包封到磷脂囊泡中的功效。
