Li Tao, Wang Yun, Wang Meng, Zheng Lingna, Dai Wanqin, Jiao Chunlei, Song Zhuda, Ma Yuhui, Ding Yayun, Zhang Zhiyong, Yang Fang, He Xiao
Hebei Provincial Key Laboratory of Green Chemical Technology & High Efficient Energy Saving, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS-HKU Joint Laboratory of Metallomics on Health & Environment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Nanomaterials (Basel). 2022 Feb 23;12(5):749. doi: 10.3390/nano12050749.
Nanoparticles (NPs) suspension is thermodynamically unstable, agglomeration and sedimentation may occur after introducing NPs into a physiological solution, which in turn affects their recognition and uptake by cells. In this work, rod-like gold NPs (AuNRs) with uniform morphology and size were synthesized to study the impact of bovine serum albumin (BSA) pre-coating on the cellular uptake of AuNRs. A comparison study using horizontal and vertical cell culture configurations was performed to reveal the effect of NPs sedimentation on AuNRs uptake at the single-cell level. Our results demonstrate that the well-dispersed AuNRs-BSA complexes were more stable in culture medium than the pristine AuNRs, and therefore were less taken up by cells. The settled AuNRs agglomerates, although only a small fraction of the total AuNRs, weighed heavily in determining the average AuNRs uptake at the population level. These findings highlight the necessity of applying single-cell quantification analysis in the study of the mechanisms underlying the cellular uptake of NPs.
纳米颗粒（NPs）悬浮液在热力学上是不稳定的，将NPs引入生理溶液后可能会发生团聚和沉淀，这反过来又会影响细胞对它们的识别和摄取。在这项工作中，合成了具有均匀形态和尺寸的棒状金纳米颗粒（AuNRs），以研究牛血清白蛋白（BSA）预包被对AuNRs细胞摄取的影响。进行了一项使用水平和垂直细胞培养配置的比较研究，以揭示NPs沉淀对单细胞水平上AuNRs摄取的影响。我们的结果表明，分散良好的AuNRs-BSA复合物在培养基中比原始AuNRs更稳定，因此细胞对其摄取较少。沉降的AuNRs聚集体，尽管仅占总AuNRs的一小部分，但在确定群体水平上AuNRs的平均摄取量方面却起着重要作用。这些发现突出了在研究NPs细胞摄取机制时应用单细胞定量分析的必要性。
