Department of Environmental Biotechnology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Sloneczna St. 45G, 10-709 Olsztyn, Poland.
Faculty of Chemistry, Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego St. 116, 90-924 Lodz, Poland.
Int J Environ Res Public Health. 2022 Feb 25;19(5):2709. doi: 10.3390/ijerph19052709.
This study investigated the effect of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) conditions (extraction time, acetone concentration, solid-to-solvent ratio) on the efficiency of polyphenol recovery from distillery stillage and antioxidant activity of the extracts. The highest total polyphenol content, flavonoid content, and phenolic acid content were obtained with 10-min UAE and 5-min MAE at a solid-to-acetone ratio of 1:15 (:). Recovery yield was the highest with an aqueous solution of 60% acetone, confirming the results of Hansen Solubility Parameter analysis. Although UAE resulted in approximately 1.2 times higher extraction yield, MAE showed a better balance between extraction yield and energy consumption exhibited by its 3-fold higher extraction rate than that of UAE. Content of total polyphenols and phenolic acids strongly correlated with antioxidant activity, indicating that these compounds provide a substantial contribution to the bioactive properties of the extracts. Six phenolic acids were extracted, predominately ferulic and p-coumaric acids, and free forms of these acids constituted 91% of their total content, which opens various possibilities for their application in the food, cosmetics, and pharmaceutical industries.
本研究探讨了超声辅助提取（UAE）和微波辅助提取（MAE）条件（提取时间、丙酮浓度、固液比）对从酿酒废料中多酚回收效率和提取物抗氧化活性的影响。在固液比为 1:15（:）下，采用 10 分钟的 UAE 和 5 分钟的 MAE，可获得最高的总多酚含量、类黄酮含量和酚酸含量。采用 60%丙酮的水溶液，回收率最高，这与 Hansen 溶解度参数分析的结果一致。尽管 UAE 的提取产率约高 1.2 倍，但 MAE 表现出更好的提取产率与能耗之间的平衡，其提取速率是 UAE 的 3 倍。总多酚和酚酸的含量与抗氧化活性强烈相关，表明这些化合物对提取物的生物活性特性有很大的贡献。提取出了六种酚酸，主要是阿魏酸和对香豆酸，这些酸的游离形式构成了它们总含量的 91%，这为它们在食品、化妆品和制药行业的应用开辟了各种可能性。
