van der Straten A, Loriau R, Herzog A, Bollen A
Biosci Rep. 1986 Apr;6(4):363-73. doi: 10.1007/BF01116423.
Various constructions of human haptoglobin (Hp) cDNA coding either for the complete alpha 2FS beta precursor protein or only for the beta subunit have been placed under the control of the lambda PR promoter in the bacterial expression vector pCQV2 (Queen, 1983). In addition to the expected 45,000 dalton polypeptide synthesized after induction of the PR promoter, the complete alpha 2FS beta constructions constitutively express a smaller polypeptide of approximately 30,000 dalton corresponding to a truncated Hp protein. Computer analysis of the HpcDNA revealed the presence of two strong potential bacterial promoters (alpha 2 PF and alpha 2 PS) located in the duplicated alpha 2FS sequence. Both Hp promoter signals are followed by potential mRNA start sites and ribosome binding sites at a compatible distance from initiation codons. In addition, the Hp alpha 2 cDNA sequence, when fused upstream to the cDNA coding for alpha 1-antitrypsin, constitutively promotes in vivo the efficient expression of an hybrid protein specifically recognized by antibodies raised against alpha 1-antitrypsin or haptoglobin.
已将编码完整α2FSβ前体蛋白或仅编码β亚基的人触珠蛋白（Hp）cDNA的各种构建体置于细菌表达载体pCQV2中的λPR启动子控制之下（奎因，1983年）。除了诱导PR启动子后合成的预期45,000道尔顿多肽外，完整的α2FSβ构建体还组成型表达一种约30,000道尔顿的较小多肽，对应于一种截短的Hp蛋白。对Hp cDNA的计算机分析显示，在重复的α2FS序列中存在两个强大的潜在细菌启动子（α2PF和α2PS）。两个Hp启动子信号后面都跟着潜在的mRNA起始位点和核糖体结合位点，它们与起始密码子的距离合适。此外，当Hpα2 cDNA序列与编码α1-抗胰蛋白酶的cDNA上游融合时，可在体内组成型促进一种杂交蛋白的高效表达，该杂交蛋白可被针对α1-抗胰蛋白酶或触珠蛋白产生的抗体特异性识别。
