Department of Ophthalmology, Hôpital Robert Debré, Reims, France.
Department of Pathology, Hôpital Robert Debré, Reims, France.
Eye (Lond). 2023 Mar;37(4):638-643. doi: 10.1038/s41433-022-01968-w. Epub 2022 Mar 10.
Altered glucose metabolism, along with low-grade inflammation, has been proposed to be involved in retinal detachment (RD)-induced cone loss. Here, we assessed intravitreal glucose and cytological profile in patients with macula-off RD.
Glucose concentration was analysed in vitreous samples from 137 non-diabetic patients undergoing vitrectomy for either primary macula-off RD (n = 73) or epiretinal membrane (ERM; n = 64). Cellularity was assessed in vitreous cytospin preparations by a semi-quantitative immunostaining approach.
Intravitreal glucose concentration was higher in the RD group (2.28 mmol.L-1 n =73 vs 1.6 mmol.L-1 n = 64; p < 0.0001). Overall cellularity and density of macrophages were significantly higher in the vitreous of RD patients (respectively p = 0.003 and p < 0.0001). Among the RD patients, intravitreal glucose concentration correlated with macrophages density (p = 0.002): its levels remained significantly higher in eyes in which macrophages were innumerable compared to lower macrophages densities RD eyes (p = 0.0095).
We observed a strong relationship between intravitreal glucose concentration and vitreous macrophage density. Additional indicators for vitreous glycation and low-grade inflammation should be further studied.
已提出改变的葡萄糖代谢以及低度炎症与视网膜脱离（RD）引起的锥体丧失有关。在这里，我们评估了黄斑裂孔 RD 患者的眼内葡萄糖和细胞学特征。
对 137 名因原发性黄斑裂孔 RD（n=73）或视网膜前膜（ERM；n=64）接受玻璃体切除术的非糖尿病患者的玻璃体样本进行了葡萄糖浓度分析。通过半定量免疫染色方法评估玻璃体离心涂片的细胞密度。
RD 组的眼内葡萄糖浓度更高（2.28mmol.L-1，n=73 与 1.6mmol.L-1，n=64；p<0.0001）。RD 患者的玻璃体总体细胞密度和巨噬细胞密度均明显更高（分别为 p=0.003 和 p<0.0001）。在 RD 患者中，眼内葡萄糖浓度与巨噬细胞密度相关（p=0.002）：与巨噬细胞密度较低的 RD 眼相比，巨噬细胞数量众多的 RD 眼的眼内葡萄糖浓度仍然明显更高（p=0.0095）。
我们观察到眼内葡萄糖浓度与玻璃体巨噬细胞密度之间存在很强的关系。应进一步研究玻璃体糖化和低度炎症的其他指标。
