Application of a Cascaded Nanozyme in Infected Wound Recovery of Diabetic Mice.

The emergence of peroxidase (POD)-like nanozyme-derived catalytic therapy has provided a promising choice for reactive oxygen species (ROS)-mediated broad-spectrum antibacterials to replace antibiotics, but it still suffers from limitations of low therapeutic efficiency and unusual addition of unstable HO. Considering that the higher blood glucose in diabetic wounds provides much more numerous nutrients for bacterial growth, a cascade nanoenzymatic active material was developed by coating glucose oxidase (GOx) onto POD-like Fe(MoO) [Fe(MoO)@GOx]. GOx could consume the nutrient of glucose to produce gluconic acid (weakly acidic) and HO, which could be subsequently converted into highly oxidative OH via the catalysis of POD-like Fe(MoO). Accordingly, the synergistic effect of starvation and ROS-mediated therapy showed significantly efficient antibacterial effect while avoiding the external addition of HO that affects the stability and efficacy of the therapy system. Compared with the bactericidal rates of 46.2-59.404% of GOx or Fe(MoO) alone on extended-spectrum β-lactamases producing and methicillin-resistant , those of the Fe(MoO)@GOx group are 98.396 and 98.776%, respectively. Animal experiments showed that the as-synthesized Fe(MoO)@GOx could much efficiently promote the recovery of infected wounds in type 2 diabetic mice while showing low cytotoxicity .