Inoue Makoto, Tanaka Yuichi, Matsushita Sakiko, Shimozaki Yuri, Ayame Hirohito, Akutsu Hidenori
Research & Business Development Center, Converting Business Development Unit, Dai Nippon Printing Co., 250-1, Wakashiba, Kashiwa 277-0871, Chiba, Japan.
Medical & Healthcare Division, Dai Nippon Printing Co., 1-1-1, Ichigaya-Kagacho, Shinjuku 162-8001, Tokyo, Japan.
Nutrients. 2022 Jan 19;14(3):438. doi: 10.3390/nu14030438.
Since many nutrients, including the three major ones of glucose, dipeptides, and cholesterol, are mainly absorbed in the small intestine, the assessment of their effects on intestinal tissue is important for the study of food absorption. However, cultured intestinal cell lines, such as Caco-2 cells, or animal models, which differ from normal human physiological conditions, are generally used for the evaluation of intestinal absorption and digestion. Therefore, it is necessary to develop an alternative in vitro method for more accurate analyses. In this study, we demonstrate inhibitory effects on nutrient absorption through nutrient transporters using three-dimensional xenogeneic-free human intestinal organoids (XF-HIOs), with characteristics of the human intestine, as we previously reported. We first show that the organoids absorbed glucose, dipeptide, and cholesterol in a transporter-dependent manner. Next, we examine the inhibitory effect of natural ingredients on the absorption of glucose and cholesterol. We reveal that glucose absorption was suppressed by epicatechin gallate or nobiletin, normally found in green tea catechin or citrus fruits, respectively. In comparison, cholesterol absorption was not inhibited by luteolin and quercetin, contained in some vegetables. Our findings highlight the usefulness of screening for the absorption of functional food substances using XF-HIOs.
由于包括葡萄糖、二肽和胆固醇这三大营养素在内的许多营养物质主要在小肠中被吸收，因此评估它们对肠道组织的影响对于食物吸收的研究很重要。然而，通常使用与正常人类生理状况不同的培养肠道细胞系，如Caco-2细胞，或动物模型来评估肠道吸收和消化。因此，有必要开发一种替代的体外方法以进行更准确的分析。在本研究中，正如我们之前所报道的，我们使用具有人类肠道特征的三维无外源基因人肠道类器官（XF-HIOs）证明了对通过营养转运蛋白的营养吸收的抑制作用。我们首先表明类器官以转运蛋白依赖性方式吸收葡萄糖、二肽和胆固醇。接下来，我们研究天然成分对葡萄糖和胆固醇吸收的抑制作用。我们发现表没食子儿茶素或川陈皮素分别对葡萄糖吸收有抑制作用，它们通常分别存在于绿茶儿茶素或柑橘类水果中。相比之下，一些蔬菜中含有的木犀草素和槲皮素对胆固醇吸收没有抑制作用。我们的研究结果突出了使用XF-HIOs筛选功能性食品物质吸收的有用性。
