Sumy State University, Sumy, Ukraine: Department of Morphology.
Sumy State University, Sumy, Ukraine: Department of Pathology.
Pol Merkur Lekarski. 2022 Feb 22;50(295):21-24.
Melanogenesis disorder is called dyschromia, is involved in various conditions including vitiligo, solar lentigo, and melanoma, the last of which is the most malignant type of skin cancer.
The aim of study was focused on histological visualization of melanogenic system.
To investigate the roles of melanocytes and melanin, we used a rat skin as an experimental animal. Samples were stained using hematoxylin-eosin, the Schmorl's technique, the Fontana-Masson technique, the Warthin-Starry technique, and the S100 immunohistochemistry marker technique. Microscopy images were analyzed using the ImageJ free software protocol.
We found Fontana-Masson staining to be the most suitable for obtaining reliable results compared with the other methods, which had more disadvantages. We also found the S100 marker staining to be an effective tool for measuring the melanocyte count.
We consider the ImageJ processing protocol and staining procedures to be useful tools to estimate melanin and melanocyte pathology.
黑色素生成障碍称为色素异常，涉及多种情况，包括白癜风、日光性雀斑和黑色素瘤，后者是最恶性的皮肤癌。
本研究的目的是专注于黑色素生成系统的组织学可视化。
为了研究黑素细胞和黑色素的作用，我们使用大鼠皮肤作为实验动物。使用苏木精-伊红、Schmorl 技术、Fontana-Masson 技术、Warthin-Starry 技术和 S100 免疫组织化学标记技术对样本进行染色。使用 ImageJ 免费软件协议分析显微镜图像。
与其他方法相比，Fontana-Masson 染色被发现是获得可靠结果的最适合方法，其他方法有更多缺点。我们还发现 S100 标记染色是测量黑素细胞计数的有效工具。
我们认为 ImageJ 处理协议和染色程序是估计黑色素和黑素细胞病理学的有用工具。
