Wang Fan, Zhao Wenjing, Chen Jiaqi, Zhou Yuhang
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China.
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China.
Chemosphere. 2022 Jul;298:134245. doi: 10.1016/j.chemosphere.2022.134245. Epub 2022 Mar 9.
The unique and efficient characteristics of allelopathy in submerged plants make it an environmentally friendly method to control harmful algal blooms. Increased research focus has been placed on the improved allelochemical extraction methods of submerged plants because of their cost-utility relationships. In this study, the growth inhibition effect of Vallisneria extract on Microcystis aeruginosa (M. aeruginosa) cells through the combination of enzyme and ultrasonic-assisted extraction method was analyzed. By establishing a co-cultivation experiment, the growth indicators, photosynthetic system, and oxidative stress system of M. aeruginosa were determined. The reactive oxygen species (ROS) and superoxide dismutase (SOD) activity, as well as the catalase (CAT) and Malondialdehyde (MDA) levels of algal cells were found to have increased significantly after co-cultivation, which indicated that the Vallisneria ultrasonic-cellulase extract could induce oxidative stress in Microcystis aeruginosa cells. The Vallisneria extract could promote at low concentrations and inhibit at high concentrations on the growth of Microcystis aeruginosa. The effective suppression of growth of algae cells with the extract was observed at 5 g/L (fresh weight). The results showed that the Vallisneria ultrasonic-cellulase extract had a significant inhibitory effect on M. aeruginosa, making the effective ingredients a useful reference for algae inhibitors.
沉水植物化感作用独特且高效的特性使其成为控制有害藻华的一种环保方法。由于成本效益关系，对沉水植物化感物质提取方法的研究关注度不断提高。本研究通过酶解与超声辅助提取相结合的方法，分析了苦草提取物对铜绿微囊藻细胞的生长抑制作用。通过建立共培养实验，测定了铜绿微囊藻的生长指标、光合系统和氧化应激系统。发现共培养后藻细胞的活性氧（ROS）和超氧化物歧化酶（SOD）活性以及过氧化氢酶（CAT）和丙二醛（MDA）水平显著增加，这表明苦草超声 - 纤维素酶提取物可诱导铜绿微囊藻细胞产生氧化应激。苦草提取物对铜绿微囊藻的生长具有低浓度促进、高浓度抑制的作用。在5 g/L（鲜重）时观察到提取物对藻细胞生长有有效抑制作用。结果表明，苦草超声 - 纤维素酶提取物对铜绿微囊藻有显著抑制作用，其有效成分可为藻类抑制剂提供有用参考。
