Department of Physiology, Faculty of Pharmacy, Institute of Nutrition and Food Technology "José Mataix", University of Granada, 18071 Granada, Spain.
Department of Physiology, Faculty of Pharmacy, Institute of Nutrition and Food Technology "José Mataix", University of Granada, 18071 Granada, Spain; Faculty of Education, Psychology and Sports Sciences, University of Huelva, 21007 Huelva, Spain.
Exp Gerontol. 2022 Jun 1;162:111766. doi: 10.1016/j.exger.2022.111766. Epub 2022 Mar 9.
BACKGROUND & AIMS: Menopausal hormonal changes increase the risk of deficiencies of minerals such as zinc (Zn), which could further worsen the decreased antioxidant defense of postmenopausal women. This study assesses the effect of 8 weeks of Zn supplementation upon the antioxidant status and clinical nutritional parameters of a postmenopausal population.
Fifty-one postmenopausal women were divided into two groups: placebo (PbG) and zinc supplementation (ZnG). Mineral status was determined by Flame Atomic Absorption Spectrophotometry (FAAS). Total Antioxidant Capacity (TAC) and Superoxide Dismutase (SOD) were analyzed by kinetic colorimetric methods. Glutathione Peroxidase (GPx) was assessed by an enzymatic immunological method.
Poor Zn status was initially observed in erythrocyte samples. Total antioxidant capacity showed a significant correlation (r = 0.730; p < 0.05) to erythrocyte Zn after the intervention (ZnG: r = 0.96; p < 0.001). Moreover, erythrocyte Zn concentration in ZnG was positively correlated to GPx activity after the intervention (r = 0.61; p < 0.01).
The postmenopausal women initially presented Zn deficiency, and the status of this mineral improved after the intervention. Zinc supplementation may be an effective approach for correcting the observed deficiencies, enhancing antioxidant defense in this risk population.
The present study is registered at the US National Institutes of Health (ClinicalTrials.gov), NCT03672513.
更年期的激素变化会增加矿物质（如锌）缺乏的风险，这可能会进一步恶化绝经后女性抗氧化防御能力的下降。本研究评估了 8 周锌补充对绝经后人群抗氧化状态和临床营养参数的影响。
51 名绝经后妇女分为两组：安慰剂组（PbG）和锌补充组（ZnG）。通过火焰原子吸收光谱法（FAAS）测定矿物质状态。总抗氧化能力（TAC）和超氧化物歧化酶（SOD）通过动力学比色法进行分析。谷胱甘肽过氧化物酶（GPx）采用酶免疫法进行评估。
初始时红细胞样本中观察到锌状态不佳。总抗氧化能力在干预后与红细胞锌呈显著相关（r=0.730；p<0.05）（ZnG：r=0.96；p<0.001）。此外，ZnG 中红细胞锌浓度与干预后 GPx 活性呈正相关（r=0.61；p<0.01）。
绝经后妇女最初表现出锌缺乏，干预后该矿物质状态得到改善。锌补充可能是纠正观察到的缺乏、增强该风险人群抗氧化防御的有效方法。
本研究在美国国立卫生研究院（ClinicalTrials.gov）注册，NCT03672513。
