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坦桑尼亚海洋和淡水鱼分离细菌的流行情况及抗菌药物耐药性

Prevalence and Antimicrobial Resistance of Bacteria Isolated from Marine and Freshwater Fish in Tanzania.

作者信息

Marijani Esther

机构信息

Open University of Tanzania, P.O. Box 23409, Dar es Salaam, Tanzania.

出版信息

Int J Microbiol. 2022 Mar 4;2022:4652326. doi: 10.1155/2022/4652326. eCollection 2022.

Abstract

This study aimed to determine the prevalence and antimicrobial resistance of bacteria isolated from retail fish and shrimp in Tanzania. A total of 92 fish and 20 shrimp samples were analyzed. Fish samples consisted of 24 Nile tilapia, 24 Nile perch, and 24 red snapper. The isolates were identified by their morphological characteristics, conventional biochemical tests, and analytical profile index test kits. The antibiotic susceptibility of selected bacteria was determined by the disc diffusion method. Out of the 92 samples analyzed, 96.7% were contaminated with 7 different bacterial species. was the most prevalent bacteria (39%), followed by spp. (28%) and spp. (16%). Other species isolated from this study were spp. (8%), (4%), spp. (3%), and s spp. (1%). All samples were analyzed for spp.; however, none of the samples tested were positive for spp. Fish from the open-air market were contaminated by six bacterial species: (40%), spp. (26%), spp. (24%), spp. (6.7%), spp. (6.5%), and spp. (2%), while (37%), spp. (33%), spp. (23%), and spp. (2%) were isolated in supermarket samples. According to the International Commission on Microbiological Specifications for Foods criteria, 54 (58.7%) and 38 (41.3%) samples were good and marginally acceptable, respectively. isolates were resistant to penicillin (PEN), erythromycin (ERY), gentamicin (GEN), azithromycin (AZM), and tetracycline (TET), while spp. isolates exhibited resistance to gentamicin (CN), tetracycline (TET), penicillin (PEN), and erythromycin (ERY). These results suggest that the presence of these bacteria might cause a health risk/hazard to human beings and may cause disease to susceptible individuals, especially immune-compromised consumers.

摘要

本研究旨在确定从坦桑尼亚零售的鱼类和虾类中分离出的细菌的流行情况和抗菌耐药性。共分析了92份鱼类样本和20份虾类样本。鱼类样本包括24份尼罗罗非鱼、24份尼罗尖吻鲈和24份红鲷鱼。通过形态特征、传统生化试验和分析谱指数测试试剂盒对分离株进行鉴定。采用纸片扩散法测定所选细菌的抗生素敏感性。在分析的92份样本中,96.7%被7种不同的细菌污染。 是最常见的细菌(39%),其次是 属(28%)和 属(16%)。本研究中分离出的其他菌种有 属(8%)、 (4%)、 属(3%)和 属(1%)。对所有样本进行了 属检测;然而,所有检测样本均未检测出 属呈阳性。露天市场的鱼类被六种细菌污染: (40%)、 属(26%)、 属(24%)、 属(6.7%)、 属(6.5%)和 属(2%),而超市样本中分离出 (37%)、 属(33%)、 属(23%)和 属(2%)。根据国际食品微生物规范委员会的标准,分别有54份(58.7%)和38份(41.3%)样本质量良好和勉强可接受。 分离株对青霉素(PEN)、红霉素(ERY)、庆大霉素(GEN)、阿奇霉素(AZM)和四环素(TET)耐药,而 属分离株对庆大霉素(CN)、四环素(TET)、青霉素(PEN)和红霉素(ERY)耐药。这些结果表明,这些细菌的存在可能对人类健康构成风险/危害,并可能导致易感个体发病,尤其是免疫功能低下的消费者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa5c/8916898/6b2c7a1f58ca/ijmicro2022-4652326.001.jpg

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