Alkahtani Saad, Hasnain Md Saquib, Algamdy Hamzah, Aljarba Nada H, AlKahtane Abdullah
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Department of Pharmacy, Palamau Institute of Pharmacy, Chianki, Daltonganj 822102, Jharkhand, India.
Saudi J Biol Sci. 2022 Mar;29(3):1585-1591. doi: 10.1016/j.sjbs.2021.11.005. Epub 2021 Nov 11.
Through the boost of the natural medicinal market, individuals began to use a variety of organic materials in the marketed herbal preparation. (LS) leaves are known as banaba. People have been using a decoction of LS leaves as antidiabetic. The study aimed to investigate the acute and sub-acute oral toxicity of LS in Sprague-Dawley rats. The acute toxicity was determined by a single oral dose of LS (2000 mg/kg). Therein animal behaviour and mortality rate were observed for 14 days. The LS (200 mg/kg) was given for 28 days daily in the sub-acute study. The body weight, organ weight, food, water intake, biochemical, haematological parameters, and histopathology were studied. The findings of this study showed no mortality or morbidity was found in acute and sub-acute toxicity studies in rats. Additionally, no significant variations were found in the respective weight of organ, haematological and biochemical parameters of treated groups with reference to the control group. Moreover, no visible histological changes were detected in the liver of treated groups with reference to the control. In conclusion, the oral administration of LS did not fabricate any major toxic effect in rats. No toxic consequences were reported during acute and sub-acute toxicity investigations. Overall, LS is a safe, natural bio-actives as studied. Further investigations of cytotoxicity and genotoxicity of the above drug(s) or their combinations may be executed for appreciative safety.
随着天然药物市场的蓬勃发展，人们开始在市售草药制剂中使用各种有机材料。（LS）叶被称为巴拿巴叶。人们一直将LS叶的煎剂用作抗糖尿病药物。本研究旨在调查LS对Sprague-Dawley大鼠的急性和亚急性口服毒性。通过单次口服剂量的LS（2000毫克/千克）来测定急性毒性。在此期间，观察动物行为和死亡率14天。在亚急性研究中，每天给予LS（200毫克/千克），持续28天。研究了体重、器官重量、食物、水摄入量、生化、血液学参数和组织病理学。本研究结果表明，在大鼠的急性和亚急性毒性研究中未发现死亡或发病情况。此外，与对照组相比，治疗组的器官重量、血液学和生化参数各自均未发现显著差异。而且，与对照组相比，治疗组的肝脏未检测到明显的组织学变化。总之，口服LS对大鼠未产生任何重大毒性作用。在急性和亚急性毒性研究期间未报告有毒后果。总体而言，如研究所示，LS是一种安全的天然生物活性物质。为了更全面地评估安全性，可对上述药物或其组合进行细胞毒性和遗传毒性的进一步研究。
