Abid Rizwana, Mahmood Riaz
Department of PG Studies and Research in Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shankaraghatta, Shivamogga, Karnataka, India.
Avicenna J Phytomed. 2019 Mar-Apr;9(2):117-125.
The plant L. (Caesalpiniaceae) is traditionally used to treat heart diseases, abdominal pain and fever. The present study was aimed to investigate the toxic effects acute and sub-acute administration of ethanol extract of fruit (CFE) in male Wistar rats.
In acute toxicity, the effects of a single oral dose (1000, 3000 and 5000 mg/kg) of CFE have been determined. Animal behaviour and mortality were determined for up to 14 days. In sub-acute study, the effects of CFE in daily single oral administration at the doses of 250, 500 and 1000 mg/kg during 28 days were determined. The blood haematological and biochemical parameters, as well as the histopathological examination of the liver, heart and kidneys were studied.
In acute study, a single administration of the CFE up to a dose of 5000 mg/kg did not induce mortality. Thus, the LD of the CFE has been estimated higher than 5000 mg/kg. In sub-acute toxicity study, administration of CFE at the doses of 250, 500 and 1000 mg/kg to rats did not induce mortality. No significant differences were found in relative organ weight, and haematological and biochemical analyses in treated groups compared to control group. No noticeable histological changes were observed in organs of CFE-treated rats compared to controls.
These results have shown that oral administration of fruit did not produce any significant toxic effect in male rats. Hence, fruit could be regarded as a safe natural product for therapeutic use.
植物L.（豆科）传统上用于治疗心脏病、腹痛和发热。本研究旨在调查果实乙醇提取物（CFE）对雄性Wistar大鼠急性和亚急性给药的毒性作用。
在急性毒性实验中，测定了单次口服剂量（1000、3000和5000mg/kg）的CFE的作用。观察动物行为和死亡率长达14天。在亚急性研究中，测定了在28天内每天单次口服给药剂量为250、500和1000mg/kg的CFE的作用。研究了血液学和生化参数，以及肝脏、心脏和肾脏的组织病理学检查。
在急性研究中，单次给予高达5000mg/kg剂量的CFE未引起死亡。因此，CFE的LD估计高于5000mg/kg。在亚急性毒性研究中，给予大鼠250、500和1000mg/kg剂量的CFE未引起死亡。与对照组相比，治疗组的相对器官重量、血液学和生化分析未发现显著差异。与对照组相比，CFE处理大鼠的器官未观察到明显的组织学变化。
这些结果表明，口服果实对雄性大鼠未产生任何显著的毒性作用。因此，果实可被视为一种安全的天然治疗用产品。
