Kumar Sandeep, Abass Ahanger Mohammad, Alshaya Huda, Latief Jan Basit, Yerramilli Vimala
Department of Botany, Chaudhary Charan Singh University, Meerut, Uttar Pradesh 250004, India.
College of Life Sciences, Northwest A&F University, China.
Saudi J Biol Sci. 2022 Mar;29(3):1337-1347. doi: 10.1016/j.sjbs.2022.01.028. Epub 2022 Jan 17.
Abiotic stress causes extensive loss to agricultural yield production worldwide. Salt stress is one of them crucial factor which leads to decreased the agricultural production through detrimental effect on growth and development of crops. In our study, we examined the effect of a defense growth substance, salicylic acid (SA 1 mM) on mature vegetative (60 Days after sowing) and flowering (80 DAS) stage of Pusa Sadabahar (PS) variety of L. plants gown under different concentrations of NaCl (25, 50, 75, 100 and 150 mM) and maintained in identical sets in pots during the whole experiment. Physiological studies indicated that increase in root & shoot length, fresh & dry weight, number of branches per plant, and yield (number of fruits per plant) under salt + SA treatment. Biochemical studies, enzymatic antioxidants like CAT, POX, and non-enzymatic antioxidant such as ascorbic acid (AsA content), carotenoids, phenolics, besides other defense compounds like proline, protein, chlorophyll contents were studied at 10 days after treatment at the mature vegetative and flowering stage. The addition of SA led to lowering of in general, all studied parameters in the mature vegetative stage but increased the same during the flowering stage, especially in the presence of NaCl; although the control I (without SA and NaCl) remained lower in value than control II (with SA, without NaCl). Interestingly, total phenolics were higher in control I (without SA or NaCl) whereas chlorophylls were higher in treatments with SA and NaCl. Thus, physiological concentration of SA (1 mM) appears to be significantly effective against salt stress during the flowering stage. In addition, during the mature vegetative stage, however, proline accumulates in SA treated sets, to help in developing NaCl-induced drought stress tolerance.
非生物胁迫给全球农业产量造成了巨大损失。盐胁迫是其中一个关键因素,它通过对作物生长发育产生不利影响,导致农业产量下降。在我们的研究中,我们研究了一种防御性生长物质水杨酸(SA,1 mM)对种植于不同浓度NaCl(25、50、75、100和150 mM)下的番茄品种Pusa Sadabahar(PS)成熟营养期(播种后60天)和花期(播种后80天)的影响,并且在整个实验过程中将其置于花盆中相同的环境条件下。生理学研究表明,在盐胁迫+SA处理下,根和茎的长度、鲜重和干重、单株分枝数以及产量(单株果实数)均有所增加。生化研究方面,在成熟营养期和花期处理10天后,对酶促抗氧化剂如CAT、POX以及非酶促抗氧化剂如抗坏血酸(AsA含量)、类胡萝卜素、酚类物质,以及其他防御性化合物如脯氨酸、蛋白质、叶绿素含量进行了研究。SA的添加总体上导致成熟营养期所有研究参数降低,但在花期有所增加,尤其是在存在NaCl的情况下;尽管对照I(无SA和NaCl)的值仍低于对照II(有SA,无NaCl)。有趣的是,对照I(无SA或NaCl)中的总酚含量较高,而SA和NaCl处理中的叶绿素含量较高。因此,SA的生理浓度(1 mM)在花期似乎对盐胁迫具有显著的抵抗作用。此外,在成熟营养期,脯氨酸在SA处理组中积累,有助于提高对NaCl诱导的干旱胁迫的耐受性。