Carvalho Renata Flávia, Aguiar-Perecin Margarida Lopes Rodrigues, Clarindo Wellington Ronildo, Fristche-Neto Roberto, Mondin Mateus
"Luiz de Queiroz" College of Agriculture, ESALQ, University of São Paulo, Piracicaba, Brazil.
Department of General Biology, Federal University of Viçosa, Viçosa, Brazil.
Front Genet. 2022 Feb 24;12:799681. doi: 10.3389/fgene.2021.799681. eCollection 2021.
Maize flowering time is an important agronomic trait, which has been associated with variations in the genome size and heterochromatic knobs content. We integrated three steps to show this association. Firstly, we selected inbred lines varying for heterochromatic knob composition at specific sites in the homozygous state. Then, we produced homozygous and heterozygous hybrids for knobs. Second, we measured the genome size and flowering time for all materials. Knob composition did not affect the genome size and flowering time. Finally, we developed an association study and identified a knob marker on chromosome 9 showing the strongest association with flowering time. Indeed, modelling allele substitution and dominance effects could offer only one heterochromatic knob locus that could affect flowering time, making it earlier rather than the knob composition.
玉米开花时间是一个重要的农艺性状，它与基因组大小和异染色质结含量的变化有关。我们整合了三个步骤来展示这种关联。首先，我们选择了在纯合状态下特定位点的异染色质结组成不同的自交系。然后，我们为这些结产生了纯合和杂合的杂种。其次，我们测量了所有材料的基因组大小和开花时间。结的组成并不影响基因组大小和开花时间。最后，我们开展了一项关联研究，并在9号染色体上鉴定出一个与开花时间关联最强的结标记。事实上，对等位基因替代和显性效应进行建模只能提供一个可能影响开花时间的异染色质结位点，使其提前开花，而不是结的组成。
